000			02516nam a22003017a 4500
003			OSt
005			20240305192412.0
008			220317b |||||||| |||| 00| 0 eng d
020			_a0306475952
020			_a9780306475955
040			_cmanual
100			_a J.N. Housby _933682
245			_aMass Spectrometry and Genomic Analysis
250			_aillustrated
260			_aLondon _b Springer Science & Business Media _c2006
300			_a 149 pages
490			_v Volume 2 of Focus on Structural Biology
520			_a76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5. Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81 5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86 6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8. Future Perspectives 90 9. Acknowledgements 91 10. References 91 CHAPTER 7 WV. Bienvenut, M. Müller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass Spectrometry: Some Aspects and Recent Developments 1. Introduction to Proteomics 93 2. Protein Biochemical and Chemical Processing Followed by Mass Spectrometric Analysis 94 2. 1. 2-DE Gel Protein Separation 95 Protein Identification Using Peptide Mass Fingerprinting and Robots 96 2. 2. 2. 2. 1. MALDI-MS Analysis 98 2. 2. 2. MS/MS Analysis 102 Improvement of the Identification by Chemical Modification of Peptides 106 2. 2. 3.
600			_xScience / Spectroscopy & Spectrum Analysis _933683
600			_xScience / Life Sciences / Zoology / General _933481
600			_xScience / Life Sciences / General _927324
600			_xScience / Life Sciences / Biophysics _926928
600			_xScience / Life Sciences / Biochemistry _927147
600			_xScience / Chemistry / Analytic _933631
600			_x Medical / Genetics _926881
600			_x Science › Life Sciences › Zoology › General _930882
942			_2ddc _cEB
999			_c6114 _d6114