000			03460nam a22003017a 4500
001			20241107162354.0
003			OCoLC
005			20241107162933.0
008			241107b |||||||| |||| 00| 0 eng d
020			_a978-1-0716-3514-8
020			_a978-1-0716-3515-5 (eBook)
022			_a 1064-3745
022			_a 1940-6029 (electronic)
040			_cddc
041			_aEnglish
100			_qLaura Miozzi
222			_aViral Metagenomics Methods and Protocols _badapter Add agarose alignment amplification analysis annotation archaeal barcode beads begomovirus Bioinformatics BLAST Botrytis cinerea buffer cDNA centrifuge Citrus tristeza virus containing contigs CRISPR database DNA DNase dsRNA elution ethanol ExTaxsI fasta fastq Fig filter flow cell fungi gel gene high-throughput sequencing host HTS IDBA identified Illumina Incubate infecting library preparation Ligation magnetic metagenomics Methods Microbiol microbiome microcentrifuge mL tube molecular mycovirus mycoviruses nanopore sequencing NCBI Note novo assembly nuclease-free water Nucleic Acids nucleotide oomycete orchid Oxford Nanopore Technologies pathogens PCR pellet pH phage Phytophthora pipeline pipette plant viruses Plasmopara primer protein protocol Qiagen Qubit reaction read mapping resuspend RNA extraction RNA-Seq room temperature rpm sample samtools small RNA solution species spin Springer Science+Business Media sRNA step sterile supernatant taxonomic tion tool total RNA Tran Integrated trimming Trinity viral viral contigs viral genomes Viral Metagenomics viral sequences viroid Virol virome ViromeScan volume Vortex µL
240			_aViral Metagenomics.
245			_aViral Metagenomics _bMethods and Protocols.
260			_aNational Research Council Bari, Italy | _b Springer Science+Business Media, LLC, part of Springer Nature | _c 2024.
300			_a294 Pages
490			_a Methods in Molecular Biology.
520			_aThis second edition volume expands on the previous edition with discussions about the latest viral metagenomics aspects covering a range of different specimens such as soil, freshwater, wastewater, fecal samples, blood plasma, clinical tissues, fungi, and herbarium samples. Chapters also look at different viral groups including archaeal viruses, eukaryotic viruses, phages, mycoviruses, and circular DNA viruses. Techniques required for studying the three viral metagenomic steps of samples processing, library construction, and analysis of data are also discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Vital Metagenomics: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this important and developing field.
600			_x Biomaterials, Genomics, Medical / Microbiology, Nucleic acids, Science / Chemistry / General, Science / Life Sciences / Genetics & Genomics, Science / Life Sciences / Microbiology, Technology & Engineering / Materials Science / General, Virology, Metagenomics -- Laboratory manuals, Viral genetics -- Laboratory manuals.
700			_qVitantonio Pantaleo.
856			_uhttps://doi.org/10.1007/978-1-0716-3515-5
942			_2ddc _cEB _n0
999			_c29729 _d29729