000			03434nam a22004097a 4500
001			20240612131137.0
003			20240612131137.0
005			20240612133611.0
008			240612b |||||||| |||| 00| 0 eng d
022			_a1471-2334
040			_cddc
041			_aEnglish
100			_qIvan Sserwadda
245			_aUnraveling virulence determinants in extended-spectrum beta-lactamase-producing Escherichia coli from East Africa using whole-genome sequencing
260			_aMwanza, Tanzania : _b Catholic University of Health and Allied Sciences [CUHAS-Bugando] : _c2023
300			_aPages 01-10
300			_aIncludes References
490			_vBMC Infectious Diseases volume 23, Article number: 587 (2023)
520			_aAbstract : Escherichia coli significantly causes nosocomial infections and rampant spread of antimicrobial resistance (AMR). There is limited data on genomic characterization of extended-spectrum β-lactamase (ESBL)-producing E. coli from African clinical settings. This hospital-based longitudinal study unraveled the genetic resistance elements in ESBL E. coli isolates from Uganda and Tanzania using whole-genome sequencing (WGS). A total of 142 ESBL multi-drug resistant E. coli bacterial isolates from both Tanzania and Uganda were sequenced and out of these, 36/57 (63.1%) and 67/85 (78.8%) originated from Uganda and Tanzania respectively. Mutations in RarD, yaaA and ybgl conferring resistances to chloramphenicol, peroxidase and quinolones were observed from Ugandan and Tanzanian isolates. We reported very high frequencies for blaCTX−M−15 with 11/18(61.1%), and blaCTX−M−27 with 12/23 (52.1%), blaTEM−1B with 13/23 (56.5%) of isolates originating from Uganda and Tanzania respectively all conferring resistance to Beta-lactam-penicillin inhibitors. We observed chloramphenicol resistance-conferring gene mdfA in 21/23 (91.3%) of Tanzanian isolates. Extraintestinal E. coli sequence type (ST) 131 accounted for 5/59 (8.4%) of Tanzanian isolates while enterotoxigenic E. coli ST656 was reported in 9/34 (26.4%) of Ugandan isolates. Virulence factors originating from Shigella dysenteriae Sd197 (gspC, gspD, gspE, gspF, gspG, gspF, gspH, gspI), Yersinia pestis CO92 (irp1, ybtU, ybtX, iucA), Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (csgF and csgG), and Pseudomonas aeruginosa PAO1 (flhA, fliG, fliM) were identified in these isolates. Overall, this study highlights a concerning prevalence and diversity of AMR-conferring elements shaping the genomic structure of multi-drug resistant E. coli in clinical settings in East Africa. It underscores the urgent need to strengthen infection-prevention controls and advocate for the routine use of WGS in national AMR surveillance and monitoring programs. .
600			_xExtended-spectrum β-lactamase
600			_x East Africa
600			_xWhole-genome sequencing
600			_xAntimicrobial resistance
600			_xVirulence factors
700			_qBenson R. Kidenya
700			_qStephen Kanyerezi
700			_qInyasi Lawrence Akaro
700			_qBaraka Mkinze
700			_qStephen E. Mshana
700			_qSuhaila O. Hashim
700			_qEverlyne Isoe
700			_q Jeremiah Seni
700			_qMoses L. Joloba
700			_qGerald Mboowa
856			_uhttps://doi.org/10.1186/s12879-023-08579-0 _yhttps://doi.org/10.1186/s12879-023-08579-0
942			_2ddc _cVM _n0
999			_c28094 _d28094