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022			_aEISSN 2076-0817
040			_cddc
041			_aEnglish
100			_qShukrani Phillip
245			_aMolecular Characterizations of the Coagulase-Negative Staphylococci Species Causing Urinary Tract Infection in Tanzania _b A Laboratory-Based Cross-Sectional Study
260			_aMwanza, Tanzania : _bCatholic University of Health and Allied Sciences [CUHAS-Bugando] : _c2023
300			_aPages 01-12
300			_aIncludes References
490			_vPathogens 2023, 12, 180.
520			_aAbstract : Background: There is a growing body of evidence on the potential involvement of coagulase-negative Staphylococci (CoNS) in causing urinary tract infections (UTIs). The aim of this study was to delineate virulence potential, antimicrobial resistance genes, and sequence types of CoNS isolated from patients with UTI symptoms and pyuria in Tanzania. Methods: CoNS from patients with UTI symptoms and more than 125 leucocytes/μL were retrieved, subcultured, and whole-genome sequenced. Results: Out of 65 CoNS isolates, 8 species of CoNS were identified; Staphylococcus haemolyticus, n = 27 (41.5%), and Staphylococcus epidermidis, n = 24 (36.9%), were predominant. The majority of S. haemolyticus were sequence type (ST) 30, with 8 new ST138-145 reported, while the majority of S. epidermidis were typed as ST490 with 7 new ST1184-1190 reported. Sixty isolates (92.3%) had either one or multiple antimicrobial resistance genes. The most frequently detected resistance genes were 53 (21%) dfrG, 32 (12.9%) blaZ, and 26 (10.5%) mecA genes conferring resistance to trimethoprim, penicillin, and methicillin, respectively. Out of 65 isolates, 59 (90.8%) had virulence genes associated with UTI, with a predominance of the icaC 47 (46.5%) and icaA 14 (13.9%) genes. Conclusion: S. haemolyticus and S. epidermidis harboring icaC, dfrG, blaZ, and mecA genes were the predominant CoNS causing UTI in Tanzania. Laboratories should carefully interpret the significant bacteriuria due to CoNS in relation to UTI symptoms and pyuria before labeling them as contaminants. Follow-up studies to document the outcome of the treated patients is needed to add more evidence that CoNS are UTI pathogens.
600			_xS. epidermidis
600			_x S. haemolyticus
600			_xicaC virulence genes
600			_xgenes for AMR
700			_qMartha F. Mushi
700			_qArun Gonzales Decano
700			_qJeremiah Seni
700			_qBlandina T. Mmbaga
700			_qHappiness Kumburu
700			_qEveline T. Konje
700			_qJoseph R. Mwanga
700			_qBenson R. Kidenya
700			_qBetrand Msemwa
700			_qStephen Gillespie
700			_qAntonio Maldonado-Barragan
700			_qAlison Sandeman
700			_qWilber Sabiti
700			_qMathew T. G. Holden
700			_qStephen E. Mshana
856			_uhttps://doi.org/10.3390/pathogens12020180 _yhttps://doi.org/10.3390/pathogens12020180
942			_2ddc _cVM _n0
999			_c27988 _d27988