000			03489nam a22003257a 4500
001			CRECU/2273 03.05.2022
003			OSt
005			20240305193956.0
008			221213b |||||||| |||| 00| 0 eng d
028			_b Phone: +255 28 298 3384
028			_b Fax: +255 28 298 3386
028			_b Email: vc@bugando.ac.tz
028			_b Website: www.bugando.ac.tz
040			_bEnglish _cDLC
041			_aEnglish
041			_aKiswahili
100			_aVivian Isaac Minja _dCUHAS/BP/3000549/T/18 _947281
210			_2I VIVIAN ISAAC MINJA hereby declare that the research work presented for the award of Bachelor in Pharmacy Degree is my original work and that it has not been submitted for any award at Catholic University of Health and Allied Sciences or other institutions _aABBREVIATIONS BMC Bugando Medical Centre CUHAS Catholic University of Health and Allied Sciences MCA Macconkey Agar MHA Mueller Hinton Agar WHO World Health Organization
222			_aOPERATIONAL DEFINITIONS _bLemon peel extract a preparation made when lemon peel is soaked in ethanol
245			_aIn Vitro susceptibility of Pseudomonas aeruginosa to crude lemon peel extract at Bugando Medical Centre, Mwanza
260			_aMwanza, Tanzania: _b Catholic University of Health and Allied Sciences [CUHAS – Bugando] _c ©03.05.2022
300			_ax; 27 Pages
300			_aIncludes Refferences and Appendices
520			_aABSTRACT: Background: Pseudomonas aeruginosa and Acinetobacter baumannii are opportunistic pathogen that causes many diseases in humans and are among main causes of hospital acquired infections .These pathogen can cause different infections in blood, lungs and the parts of the body This pathogen showed resistance to many drugs including the drugs that used to be first line in treatment like trimethoprim-sulfamethoxazole, aminoglycosides, cephalosporin and fluoroquinolones. This study aim at determining the susceptibility of P. aeruginosa and A. baumannii to crude lemon peel extract to help solve this problem. Methods: The laboratory based experimental study was conducted in the Microbiology and Pharmacognosy laboratories at (CUHAS) and at Tanzania Medicine and Medical Devices Authority. Lemon peel extract was prepared by cold maceration and used to test its activity against P. aeruginosa and A. baumannii clinical isolates obtained by convenient sampling stored in the laboratory from Bugando Medical Centre using well diffusion method and disc diffusion method where mean zone of inhibition were recorded. Results: Lemon peel extract showed bactericidal properties to P. aeruginosa and no activity to A. baumannii. Overall mean zone of inhibition was 9.4(±3.8) mm whereby for P. aeruginosa was 10.5(±3.8) mm and for A. baumannii 6(±0.0) mm. Conclusion: Results of this study showed that lemon peel extract from Citrus Limon showed antibacterial properties against P. aeruginosa. Plant medicinal sources are natural, cheap, relative safe, due to increase in antibiotic resistance among bacteria making them an important source of medicines. Since P. aeruginosa is among nosocomial infections causing bacteria lemon is a potential source of phytochemicals which can be used to reduce bacteria loads and treat patients
600			_x Microbiology and Immunology _927748
700			_aBetrand Msemwa _919669
700			_aVitus Silago _919665
710			_a Tekla Joseph _947453
942			_2ddc _cCR
999			_c22648 _d22648