000 04335nam a22003377a 4500
001 CUHAS/BM/1000252/T/16
003 CUHAS/BM/1000252/T/16
005 20240305193836.0
008 210806b |||||||| |||| 00| 0 eng d
028 _bWurzburg Road 35, BMC Premises, Post Code: 33102:
028 _bP. O. Box 1464, Mwanza – Tanzania:
028 _bPhone: +255 28 298 3384:
028 _b Fax: +255 28 298 3386:
028 _bEmail: vc@bugando.ac.tz :
028 _b www.bugando.ac.tz
035 _aCUHAS/BM/1000252/T/16
040 _bEnglish
_cDDC
041 _aEnglish
041 _aKiswahili
100 _a Nyanda, Boaz. M
_dCUHAS/BM/1000252/T/16
_920767
245 _a Escherichia Coli Serotype O157:H7 In Humans, Domestic Animals and in the Environmental Premises in Mwanza and Shinyanga, Tanzania.
260 _aMwanza, Tanzania:
_bCatholic University of Health and Allied Sciences [CUHAS – Bugando] :
_c©2019
300 _a vii; 43 Pages
300 _aIncludes References
520 _aAbstract: Background: Diarrhea due to Escherichia coli serotype O157:H7 is one of the leading causes of morbidity and mortality globally among enteropathogens. Previous study conducted in Mwanza Tanzania on diarrhea among under five children was based on detection of Shiga Toxin Producing Escherichia coli (STEC). This study aim to add more information (in addition of under five children with diarrhea) by including domestic and their surrounding environmental premises to determine the prevalence and drug susceptibility pattern of STEC serotype O157:H7 and STEC non O157:H7 isolates in Mwanza and Shinyanga, Tanzania. Methods: A cross sectional study was conducted involving under five children with diarrhea, domestic animals and environmental specimens between May to July 2018 and 2019 in Mwanza and Shinyanga, Tanzania. Stool samples from children with diarrhea, recto-fecal samples from domestic animals and selected environmental samples (such as juices, salad, and water) were collected and cultured on MacConkey agar and CHROMO agar to identify Escherichia coli and STEC, respectively. Then, all STEC isolates were cultured in Sorbitol MacConkey agar to identify STEC serotype O157:H7. Demographic and clinical information were collected using a standard tool and STATA software version 13.0 used for data analysis. Results: Out of 355 stool samples of under five children, 290 (86.7%) had positive culture results, where by 220 (62%) samples were positive for E. coli. Out of 220 E. coli, 45 (20.5%) were STEC positive and out 45 positive STEC, 5 (11.1%) were O157H7. Out of 285 animal specimens, 217 (76.1%) had positive culture results, where by 204 (71.6%) were positive for E. coli. Out of 204 E. coli, 52 (25.5%) were STEC positive and out of 52 STEC positive, 7 (13.5%) were O157H7. Out of 142 environmental specimens collected, 56 (39.4%) had positive culture results, where by 15 (10.6%) were positive for E. coli. Out of 15 E. coli, 5 (33.3%) were STEC positive and out of 5 STEC strains, 3 (60%) were O157H7. Children under five years of age residing in urban areas had higher STEC (28.4%) than those residing in the rural areas (17.0%), although the association was not statistically significant (p-value=0.058). STEC was significantly associated with domestic animals residing in Sengerema (20.5%) and Shinyanga (34.6%) compared to Mwanza (7.0%), P-value <0.001. STEC was also associated with chicken (22.0%) and pigs (33.3%) compared to other domestic animals (p-value=0.013) Conclusions: The proportions of STEC among E. coli strains in three interfaces is high but the proportions of E. coli O157:H7 is low. Of note, STEC strains were more associated children in urban areas, and also in chicken and pigs. Significance: This study added more information on the STEC and STEC O157:H7 as potential causes of diarrhea among children under five years of ages, and also evaluated the potential source of these pathogens among domestic animals and environmental premises. These is turn will help in the institution of specific preventive measures using One Health Approach.
600 _xMedical Laboratory Sciences
600 _xMicrobiology & Immunology
700 _a Seni, Jeremiah
_919713
700 _aFrank Van Der Meer
_920768
942 _2ddc
_cCR
999 _c20830
_d20830