000 02342nam a22002057a 4500
001 CUHAS/BP/3000193/T/14
007 Undergraduate Ethical Clearance Certificate No. 543/2018
008 210728b |||||||| |||| 00| 0 eng d
100 _a Somaiya, Sejal. P
_dCUHAS/BP/3000193/T/14
_919702
245 _a In Vitro Antifungal Activity of Ocimum Sanctum L. Leaf Extracts on Candida Albicans Clinical Isolates in Mwanza Tanzania
260 _aMwanza, Tanzania:
_bCatholic University of Health and Allied Sciences [CUHAS - Bugando] :
_bPhone: +255 28 298 3384 :
_b Fax: +255 28 298 3386 :
_b Email: vc@bugando.ac.tz :
_bWebsite: www.bugando.ac.tz :
_c ©30.08.2018
300 _a x; 22 Pages
300 _aIncludes Refferences and Appedices
520 _aAbstract: Background: Oral candidiasis is amongst the most common opportunistic fungal infections caused mainly by C. albicans. Resistance of C. albicans to the most commonly prescribed antifungal agents such as azoles have been reported even in Tanzania. This imposes the need to explore alternative medicinal products. Plants such as O. sanctum L. are an important source of herbal medicine. Methodology: Different concentrations of O. sanctum L. leaf extracts; (2.5mg/ml), (5mg/ml), (7.5mg/ml), (10mg/ml), 15mg/ml), (20mg/ml) were made in aqueous solution form and were tested against 20 C. albicans isolates. Agar dilution method using Muller Hinton Agar (MH – Oxoid, UK) supplemented with the different concentrations of the leaf extract was used to test for susceptibility against C. albicans isolates. The minimum concentration that inhibited the growth of C. albicans was considered as Minimum Inhibition concentration (MIC). Results: A total of 20(100%), 13(65%) and 9(45%) C. albicans isolates were inhibited by O. sanctum L. extract at the concentration of 20mg/ml, 15mg/ml and 10mg/ml, respectively. Our results show the minimum inhibitory concentration of O. sanctum L leaves extract is 20mg/ml. Conclusion: The study results revealed that the effectiveness of O. sanctum L. leaf extracts on pathogen C. albicans increases with the increased concentrations.
600 _xPharmacy
_926693
600 _xMicrobiology and Immunology
_948529
700 _a Okamo, Bernard
_919703
700 _a Mushi, Martha
_919704
942 _2ddc
_cCR
999 _c20085
_d20085