000			01819nam a22002897a 4500
003			OSt
005			20240305193742.0
008			221201b |||||||| |||| 00| 0 eng d
028			_b Phone: +255 28 298 3384
028			_b Fax: +255 28 298 3386
028			_b Email: vc@bugando.ac.tz
028			_b Website: www.bugando.ac.tz
040			_bEnglish _cDLC
041			_aEnglish
100			_aP Koliopoulos _946302
245			_aDetecting Plasmodium Sp and Arboviruses, All in One Test
260			_aMwanza, Tanzania: _bRomanian Archives of Microbiology and Immunology & _b Catholic University of Health and Allied Sciences [CUHAS – Bugando] _c2021
490			_vRomanian Archives of Microbiology and Immunology; Bucharest Vol. 80, Iss. 3, (Jul-Sep 2021): 298.
520			_aThe current study evaluates the ability of a multiplex-reverse transcriptase-polymerase chain reaction test combined with an enzyme linked immunosorbent assay (multiplex-RT-PCR-ELISA) to identify and differentiate nine human mosquitoborne pathogens (eight arboviruses and Plasmodium sp.). Venous blood samples were collected from 132 children (ranging from 5-months to 12-years) who presented with acute high-grade fever (> 38.0 C) and met the WHO criteria of a presumptive malaria infection, to a medical center in Tanzania, between April 2016 and October 2016. Serial dilutions of a standard Zika virus suspension (1x107 viral RNA copies/ml) were added to the master mix containing the 9 primer sets.
700			_a NM Kayange _946303
700			_a T Daniel _946304
700			_a F Huth _946305
700			_aB Gröndahl _946306
700			_aGC Medina-Montaño _946307
856			_uhttps://www.proquest.com/openview/0465855cfcc19d7123df819006c8bb43/1?pq-origsite=gscholar&cbl=4585458
942			_2ddc _cVM
999			_c19835 _d19835