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| 003 | OSt | ||
| 005 | 20240305193734.0 | ||
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| 028 | _b Phone: +255 28 298 3384 | ||
| 028 | _b Fax: +255 28 298 3386 | ||
| 028 | _b Email: vc@bugando.ac.tz | ||
| 028 | _b Website: www.bugando.ac.tz | ||
| 040 |
_bEnglish _cDLC |
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| 041 | _aEnglish | ||
| 100 |
_aDoreen Kamori _945771 |
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| 222 | _aPregnancy, Asymptomatic bacteriuria, Extended Spectrum Beta-Lactamase, Enterobacteriaceae, Resistance | ||
| 245 | _aExtended Spectrum Beta-Lactamase Producing Enterobacteriaceae Associated with Asymptomatic Bacteriuria among Pregnant Women Attending Antenatal Clinic at Tertiary Referral Hospital, Tanzania | ||
| 260 |
_aMwanza, Tanzania: _bTanzania Medical Journal & _bCatholic University of Health and Allied Sciences [CUHAS – Bugando] _c2022/1/31 |
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| 300 | _aPages 75-92 | ||
| 490 | _vTanzania Medical Journal Volume 33 Issue 1 | ||
| 520 | _a Abstract: Background: Asymptomatic bacteriuria occurs in 2-15% of pregnancies resulting in acute pyelonephritis, preterm labor, pre-eclampsia, anemia, amnionitis, low birth weight, stillbirths, bacteremia and toxic septicemia. Asymptomatic bacteriuria in pregnancy (ASBP) caused by extended-spectrum beta-lactamases producing Enterobacteriaceae (ESBL-PE) further complicates the health of a pregnant woman, affecting treatment and spread of resistant bacteria strains to newborns and the community. However, in Tanzania, screening for resistant bacteria such as ESBL-PE in ASBP is not routinely done. Broad objective: To determine the prevalence of ASBP associated with ESBL-PE and the antimicrobial susceptibility pattern of ESBL-PE isolated from pregnant women at Muhimbili National Hospital (MNH) in Dar es salaam, Tanzania. Methodology: A hospital-based cross-sectional study was conducted at MNH. A total of 182 pregnant women with the gestational age of 37 weeks and above were enrolled. A semi-structured questionnaire and antenatal cards were used to collect socio-demographic and pregnancy information. Clean catch mid-stream urine was collected for screening of asymptomatic bacteriuria. Bacteria were identified using conventional biochemical methods and antimicrobial susceptibility testing (AST) was performed by Kirby-Bauer method following Clinical Laboratory Standard Institute (CLSI) guidelines. The isolates resistant to ceftazidime and cefotaxime were confirmed for ESBL production using a double-disc synergy test (DDST). Results: Asymptomatic bacteriuria was observed in 13% (24/182) of the pregnant women attending antenatal clinic at MNH. We report that, 61.9% (13/21) of women with asymptomatic bacteriuria associated with gram negative bacteria were infected with ESBL-PE. Among the ESBL-PE species mostly isolated include E. coli (69.2%), followed by K. pneumoniae (23.1%), and K. oxytoca (7.7%); and non ESBL-PE species isolated were E. coli (87.5.6%), and K. pneumoniae (12.5%). In addition, three S. aureus isolates were detected in women with ASBP. ESBL-PE isolates showed high resistance to aztreonam, sulphamethoxazole-trimethoprim, amikacin and nalidixic acid; while for the few detected non ESBL-PE high resistance was seen to sulphamethoxazole-trimethoprim, aztreonam, meropenem and nalidixic acid. Â Conclusion and recommendation: The present study revealed that a high proportion of bacteriuria in pregnancy is associated with ESBL-PE. These findings suggest a need for screening of resistant bacteria such as ESBL in cases of ASBP. | ||
| 700 |
_a Ambele M Mwandigha _945839 |
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| 700 |
_a Upendo Kibwana _945840 |
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| 700 |
_aSalim Masoud _945841 |
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| 700 |
_a Mariam M Mirambo _922927 |
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| 700 |
_a Stephen E Mshana _915820 |
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| 700 |
_aJoel Manyahi _945576 |
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| 700 |
_a Mtebe Majigo _922970 |
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| 856 | _uhttps://doi.org/10.4314/tmj.v33i1.522 | ||
| 942 |
_2ddc _cVM |
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_c19634 _d19634 |
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