000			03249nam a22002897a 4500
003			OSt
005			20240305193732.0
008			221122b |||||||| |||| 00| 0 eng d
028			_bPhone: +255 28 298 3384
028			_bFax: +255 28 298 3386
028			_b Email: vc@bugando.ac.tz
028			_bWebsite: www.bugando.ac.tz
040			_bEnglish _cDLC
041			_aEnglish
100			_aNyambura Moremi _922727
222			_a S. aureus Colonization Surgical site infection Tanzania
245			_aThe role of patients and healthcare workers Staphylococcus aureus nasal colonization in occurrence of surgical site infection among patients admitted in two centers in Tanzania
260			_aMwanza, Tanzania: _b BioMed Central & _bCatholic University of Health and Allied Sciences [CUHAS – Bugando] _c17 June 2019
300			_aPages 1-7
490			_vAntimicrobial Resistance & Infection Control Volume 8 Issue 1
520			_aAbstract Background: Colonization with Staphylococcus aureus has been identified as a risk for subsequent occurrence of infection. This study investigated the relationship between S. aureus colonization of patients and healthcare workers (HCWs), and subsequent surgical site infections (SSI). Methods: Between December 2014 and September 2015, a total of 930 patients and 143 HCWs were enrolled from the Bugando Medical Centre and Sekou Toure hospital in Mwanza, Tanzania. On admission and discharge nasal swabs, with an additional of wound swab for those who developed SSI were collected from patients whereas HCWs were swabbed once. Identification and antimicrobial susceptibility testing were done by VITEK-MS and VITEK-2, respectively. Detection of Panton Valentine leukocidin (PVL) and mecA genes was done by PCR. S. aureus isolates were further characterized by spa typing and Multi-Locus Sequence Typing (MLST). Results: Among 930 patients screened for S. aureus on admission, 129 (13.9%) were positive of which 5.4% (7/129) were methicillin-resistant S. aureus (MRSA). Amongst 363 patients rescreened on discharge, 301 patients had been tested negative on admission of whom 29 (9.6%) turned positive after their hospital stay. Three (10.3%) of the 29 acquired S. aureus were MRSA. Inducible Clindamycin resistance occurred more often among acquired S. aureus isolates than among isolates from admission [34.5% (10/29) vs. 17.1% (22/129), P = 0.018]. S. aureus contributed to 21.1% (n = 12) of the 57 cases of investigated SSIs among 536 patients followed. Seven out of eight S. aureus carriage/infection pairs had the same spa and sequence types. The previously reported dominant PVL-positive ST88 MRSA strain with spa type t690 was detected in patients and HCW. Conclusion: A significant proportion of patients acquired S. aureus during hospitalization. The finding of more than 90% of S. aureus SSI to be of endogenous source underscores the need of improving infection prevention and control measures including screening and decolonization of high risk patients.
700			_aHeike Claus _923349
700			_aUlrich Vogel _923624
700			_a Stephen E Mshana _915820
856			_uhttps://doi.org/10.1186/s13756-019-0554-y
942			_2ddc _cVM
999			_c19552 _d19552