000			03301nam a22005057a 4500
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008			221121b |||||||| |||| 00| 0 eng d
022			_a 2045-2322 (online)
028			_b Phone: +255 28 298 3384
028			_b Fax: +255 28 298 3386
028			_b Email: vc@bugando.ac.tz
028			_b Website: www.bugando.ac.tz
040			_bEnglish _cDLC
041			_aEnglish
100			_aNiladri Bhusan Pati _945607
222			_a Bacterial evolution Bacterial genomics Bacterial pathogenesis Infectious-disease epidemiology
245			_aEnterobacter bugandensis _ba novel enterobacterial species associated with severe clinical infection
260			_aMwanza, Tanzania: _bNature Publishing Group & _b Catholic University of Health and Allied Sciences [CUHAS – Bugando] _c2018/3/29
300			_aPages 1-11
490			_v Scientific reports Volume 8 Issue 1
520			_aAbstract Nosocomial pathogens can cause life-threatening infections in neonates and immunocompromised patients. E. bugandensis (EB-247) is a recently described species of Enterobacter, associated with neonatal sepsis. Here we demonstrate that the extended spectrum ß-lactam (ESBL) producing isolate EB-247 is highly virulent in both Galleria mellonella and mouse models of infection. Infection studies in a streptomycin-treated mouse model showed that EB-247 is as efficient as Salmonella Typhimurium in inducing systemic infection and release of proinflammatory cytokines. Sequencing and analysis of the complete genome and plasmid revealed that virulence properties are associated with the chromosome, while antibiotic-resistance genes are exclusively present on a 299 kb IncHI plasmid. EB-247 grew in high concentrations of human serum indicating septicemic potential. Using whole genome-based transcriptome analysis we found 7% of the genome was mobilized for growth in serum. Upregulated genes include those involved in the iron uptake and storage as well as metabolism. The lasso peptide microcin J25 (MccJ25), an inhibitor of iron-uptake and RNA polymerase activity, inhibited EB-247 growth. Our studies indicate that Enterobacter bugandensis is a highly pathogenic species of the genus Enterobacter. Further studies on the colonization and virulence potential of E. bugandensis and its association with septicemic infection is now warranted.
700			_a Trinad Chakraborty _923153
700			_a Jörg Overmann _923572
700			_aStephen E Mshana _915820
700			_a Can Imirzalioglu _915822
700			_aTorsten Hain _923625
700			_aLinda Falgenhauer _923152
700			_aAlexander Goesmann _945608
700			_a Cathrin Spröer _945609
700			_aJulian D Hegemann _945610
700			_a Mohamed A Marahiel _945611
700			_aMobarak Abu Mraheil _945612
700			_aBoyke Bunk _945613
700			_aKonrad Gwozdzinski _945614
700			_a Mrutyunjay Suar _945615
700			_a Daniel Ryan _945616
700			_a Sangeeta Jaiswal _945617
700			_aYancheng Yao _945618
700			_a Gopala Krishna Mannala _945619
700			_a Tilman Schultze _945620
700			_aSwapnil Prakash Doijad _945621
856			_uhttps://doi.org/10.1038/s41598-018-23069-z
942			_2ddc _cVM
999			_c19541 _d19541