000			03016nam a22003257a 4500
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022			_a 1471-2334
028			_b Phone: +255 28 298 3384
028			_b Fax: +255 28 298 3386
028			_bEmail: vc@bugando.ac.tz
028			_b Website: www.bugando.ac.tz
040			_bEnglish _cDLC
041			_aEnglish
100			_aStephen E Mshana _915820
222			_a Cefepime Tigecycline Cefpodoxim ESBL Gene ESBL Type
245			_aConjugative IncFI plasmids carrying CTX-M-15 among Escherichia coliESBL producing isolates at a University hospital in Germany
260			_aMwanza, Tanzania: _b BioMed Central & _b Catholic University of Health and Allied Sciences [CUHAS – Bugando] _c17 June 2009
300			_aPages 1-8
490			_vBMC infectious diseases Volume 9 Issue 1
520			_aAbstract Background: Multi-drug-resistant, extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, constitute an emerging public-health concern. Little data on the molecular epidemiology of ESBL producing Escherichia coli is available in Germany. Here we describe the prevalence and molecular epidemiology of ESBL producing-Escherichia coli isolates at a German University hospital. Methods: We analysed 63 non-duplicate clinical ESBL isolates obtained over an 8-month period using PCR and sequence-based ESBL allele typing, plasmid replicon typing, phylogenetic group typing. Pulsed-field gel electrophoresis (PFGE) based genotyping and plasmid profiling was performed, as well as confirmatory DNA-based hybridization assays. Results: Examination of the 63 Escherichia coli isolates revealed an almost equal distribution among the E. coli phylogenetic groups A, B1, B2 and D. High prevalence (36/63) of the CTX-M-15 gene was observed and an analysis of PFGE-based patterns revealed the presence of this CTX-M allele in multiple clones. Resistance to cefotaxime was a transferable trait and a commonly occurring 145.5 kb conjugative IncFI plasmid was detected in 65% of E. coli carrying the CTX-M-15 allele. The rate of transferable antibiotic resistances for GM, SXT, TET, GM-SXT-TET, SXT-TET and GM-TET was 33%, 61%, 61%, 27%, 44% and 11%, respectively. The remaining strains did not have a common IncFI plasmid but harboured transferable IncFI plasmids with sizes that ranged from 97 to 242.5 kb. Conclusion: Our data demonstrate the presence of IncFI plasmids within the prevailing E. coli population in a hospital setting and suggest that the dissemination of CTX-M-15 allele is associated to lateral transfer of these well-adapted, conjugative IncFI plasmids among various E. coli genotypes.
700			_aCan Imirzalioglu _915822
700			_aHamid Hossain _945578
700			_aTorsten Hain _923625
700			_aEugen Domann _923626
700			_a Trinad Chakraborty _923153
856			_uhttps://doi.org/10.1186/1471-2334-9-97
942			_2ddc _cVM
999			_c19529 _d19529