000			03129nam a22003137a 4500
003			OSt
005			20240305193731.0
008			221121b |||||||| |||| 00| 0 eng d
022			_a1756-0500
028			_b Phone: +255 28 298 3384
028			_b Fax: +255 28 298 3386
028			_bEmail: vc@bugando.ac.tz
028			_b Website: www.bugando.ac.tz
040			_bEnglish _cDLC
041			_aEnglish
100			_a Stephen E Mshana _915820
222			_a Klebsiella Pneumoniae Negative Bacterium Generation Cephalosporin Blood Stream Infection ESBL Production
245			_aPrevalence of multiresistant gram-negative organisms in a tertiary hospital in Mwanza, Tanzania
260			_aMwanza, Tanzania: _b BioMed Central & _b Catholic University of Health and Allied Sciences [CUHAS – Bugando] _c26 March 2009
300			_aPages 1-6
490			_vBMC research notes Volume 2 Issue 1
520			_aAbstract Background: Antimicrobial resistance is fast becoming a global concern with rapid increases in multidrug-resistant Gram negative organisms. The prevalence of extended spectrum beta-lactamase (ESBL)-producing clinical isolates increases the burden on implementing infectious disease management in low socio-economic regions. As incidence can vary widely between regions, this study was done to determine resistance patterns of Gram-negative organisms at Bugando Medical Center, a tertiary hospital in Mwanza, Tanzania. Methods: A total of 800 clinical samples (urine, wound swab, pus, blood, aspirate, sputum etc) were processed over a period of 6 months. Gram-negative bacteria were identified using conventional in-house biochemical tests and susceptibility to common antibiotics done using disc diffusion methods. The disc approximation method was used to identify ESBL producers. Results: A total of 377 Gram-negative bacteria (GNB) recovered from 377 clinical specimens were analyzed of which 76.9% were Enterobacteriaceae. Among all GNB, 110/377 (29.2%) were found to be ESBL producers. Species specific ESBLs rate among Klebsiella pneumoniae, Escherichia coli, Acinetobacter spp, Proteus spp and other enterobacteria were 63.7%, 24.4%, 17.7%, 6.4% and 27.9% respectively. A statistically significant higher number of inpatients 100/283 (35.3%) compared to 10/94 (10.6%) of outpatients had ESBL-producing organisms (p = 0.000023). Rates of resistances to gentamicin, tetracycline, sulphamethaxazole/trimethoprim and ciprofloxacin were significantly higher among ESBLs isolates than non-ESBL isolates (p = 0.000001). Conclusion: ESBL producing organisms are common at BMC (Bugando Medical Center) and pose a challenge to antibiotic therapy. Successful implementation of a routine detection of ESBL production is essential in designing appropriate antibiotic prescribing policies and infection control intervention programmes.
700			_aErasmus Kamugisha _922814
700			_aMariam Mirambo _919596
700			_aTrinad Chakraborty _923153
700			_aEligius F Lyamuya _923627
856			_uhttps://doi.org/10.1186/1756-0500-2-49
942			_2ddc _cVM
999			_c19527 _d19527