| 000 | 02500nam a22003857a 4500 | ||
|---|---|---|---|
| 003 | OSt | ||
| 005 | 20240305193725.0 | ||
| 008 | 221110b |||||||| |||| 00| 0 eng d | ||
| 022 | _a1471-2172 | ||
| 028 | _b Phone: +255 28 298 3384 | ||
| 028 | _b Fax: +255 28 298 3386 | ||
| 028 | _b Email: vc@bugando.ac.tz | ||
| 028 | _b Website: www.bugando.ac.tz | ||
| 040 | _cDLC | ||
| 041 | _aEnglish | ||
| 100 |
_aXiaoping Zhang _945267 |
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| 222 | _a KCTD9 Short hairpin RNA NK cell Liver failure MHV-3 | ||
| 245 | _aInterference with KCTD9 inhibits NK cell activation and ameliorates fulminant liver failure in mice | ||
| 260 |
_aMwanza: _bBioMed Central & _b Tanzania Catholic University of Health and Allied Sciences [CUHAS – Bugando] _c25 June 2018 |
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| 300 | _a Pages 1-11 | ||
| 490 | _vBMC immunology Volume 19 Issue 1 | ||
| 520 | _aAbstract Background: Potassium channel tetramerisation domain containing 9 (KCTD9), a member of KCTD family with a DNA-like pentapeptide repeat domain, was found to be increased particularly in NK cells of patients with HBV-induced acute-on-chronic liver failure (HBV-ACLF) and experimental viral fulminant hepatitis. Knockdown of KCTD9 in immortalized NK cells inhibits cytokines production and cytotoxicity. As NK cell activation was shown to exacerbate liver damage in viral fulminant hepatitis, we propose that target inhibition of KCTD9 may prohibit NK cells activity and thus ameliorate liver damage in viral fulminant hepatitis. Result: Hydrodynamic delivery of plasmid expressing short-hairpin RNA against KCTD9 resulted in impaired NK cells function as demonstrated by reduced cytokine production and cytotoxicity, and ameliorated liver injury as manifested by improved liver histology and survival rate. In contrast, delivery of plasmid expressing KCTD9 led to deteriorated disease progression. Conclusion: Interference with KCTD9 expression exert beneficial effect in viral fulminant hepatitis therapy. Such effect may be mediated by impairment of NK cell activation. | ||
| 700 |
_a Lin Zhu _945268 |
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| 700 |
_a Yaoyong Zhou _945269 |
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| 700 |
_aAichao Shi _945270 |
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| 700 |
_a Hongwu Wang _945271 |
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| 700 |
_a Meifang Han _945272 |
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| 700 |
_a Xiaoyang Wan _945273 |
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| 700 |
_aSemvua Bukheti Kilonzo _922895 |
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| 700 |
_aXiaoping Luo _945274 |
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| 700 |
_aTao Chen _945275 |
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| 700 |
_a Qin Ning _945241 |
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| 856 | _uhttps://doi.org/10.1186/s12865-018-0256-x | ||
| 942 |
_2ddc _cVM |
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| 999 |
_c19386 _d19386 |
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