000			03121nam a22003137a 4500
008			210826b |||||||| |||| 00| 0 eng d
022			_a2047-2994
100			_a David Patrick Kateete _923720
222			_a Eastern Uganda; Iganga/Mayuge districts; Coexistence; Hospital-associated MRSA; Community-associated MRSA; mecA; SCCmec types; spa types
245			_a CA-MRSA and HA-MRSA coexist in community and hospital settings in Uganda
260			_aMwanza, Tanzania: _bCatholic University of Health and Allied Sciences CUHAS - Bugando & _b BioMed Central _c 03 June 2019
300			_a Pages 1-9
490			_vAntimicrobial Resistance & Infection Control Volume 8 Issue 1
520			_aAbstract Background: Methicillin resistant Staphylococcus aureus (MRSA) strains were once confined to hospitals however, in the last 20 years MRSA infections have emerged in the community in people with no prior exposure to hospitals. Strains causing such infections were novel and referred to as community-associated MRSA (CA-MRSA). The aim of this study was to determine the MRSA carriage rate in children in eastern Uganda, and to investigate coexistence between CA-MRSA and hospital-associated (HA-MRSA). Methods: Between February and October 2011, nasopharyngeal samples (one per child) from 742 healthy children under 5 years in rural eastern Uganda were processed for isolation of MRSA, which was identified based on inhibition zone diameter of ≤19 mm on 30 μg cefoxitin disk. SCCmec and spa typing were performed for MRSA isolates. Results: A total of 140 S. aureus isolates (18.9%, 140/742) were recovered from the children of which 5.7% (42/742) were MRSA. Almost all (95.2%, 40/42) MRSA isolates were multidrug resistant (MDR). The most prevalent SCCmec elements were types IV (40.5%, 17/42) and I (38.1%, 16/42). The overall frequency of SCCmec types IV and V combined, hence CA-MRSA, was 50% (21/42). Likewise, the overall frequency of SCCmec types I, II and III combined, hence HA-MRSA, was 50% (21/42). Spa types t002, t037, t064, t4353 and t12939 were detected and the most frequent were t064 (19%, 8/42) and t037 (12%, 5/42). Conclusion: The MRSA carriage rate in children in eastern Uganda is high (5.7%) and comparable to estimates for Mulago Hospital in Kampala city. Importantly, HA-MRSA (mainly of spa type t037) and CA-MRSA (mainly of spa type t064) coexist in children in the community in eastern Uganda, and due to high proportion of MDR detected, outpatient treatment of MRSA infection in eastern Uganda might be difficult.
700			_aMoses L. Joloba _923380
700			_a Benon B. Asiimwe _923721
700			_a Elizeus Rutebemberwa _923722
700			_aKarin Källander _923723
700			_a Christine F. Najjuka _923377
700			_aHannington Baluku _923724
700			_aFred K. Ashaba _923725
700			_aBrian Mujuni _923726
700			_aEdgar Kigozi _923727
700			_aRaymond Mayanja _923728
700			_aJeremiah Seni _919633
700			_a Freddie Bwanga _923376
856			_uhttps://doi.org/10.1186/s13756-019-0551-1
942			_2ddc _cVM
999			_c19025 _d19025