000 03121nam a22003137a 4500
008 210826b |||||||| |||| 00| 0 eng d
022 _a2047-2994
100 _a David Patrick Kateete
_923720
222 _a Eastern Uganda; Iganga/Mayuge districts; Coexistence; Hospital-associated MRSA; Community-associated MRSA; mecA; SCCmec types; spa types
245 _a CA-MRSA and HA-MRSA coexist in community and hospital settings in Uganda
260 _aMwanza, Tanzania:
_bCatholic University of Health and Allied Sciences CUHAS - Bugando &
_b BioMed Central
_c 03 June 2019
300 _a Pages 1-9
490 _vAntimicrobial Resistance & Infection Control Volume 8 Issue 1
520 _aAbstract Background: Methicillin resistant Staphylococcus aureus (MRSA) strains were once confined to hospitals however, in the last 20 years MRSA infections have emerged in the community in people with no prior exposure to hospitals. Strains causing such infections were novel and referred to as community-associated MRSA (CA-MRSA). The aim of this study was to determine the MRSA carriage rate in children in eastern Uganda, and to investigate coexistence between CA-MRSA and hospital-associated (HA-MRSA). Methods: Between February and October 2011, nasopharyngeal samples (one per child) from 742 healthy children under 5 years in rural eastern Uganda were processed for isolation of MRSA, which was identified based on inhibition zone diameter of ≤19 mm on 30 μg cefoxitin disk. SCCmec and spa typing were performed for MRSA isolates. Results: A total of 140 S. aureus isolates (18.9%, 140/742) were recovered from the children of which 5.7% (42/742) were MRSA. Almost all (95.2%, 40/42) MRSA isolates were multidrug resistant (MDR). The most prevalent SCCmec elements were types IV (40.5%, 17/42) and I (38.1%, 16/42). The overall frequency of SCCmec types IV and V combined, hence CA-MRSA, was 50% (21/42). Likewise, the overall frequency of SCCmec types I, II and III combined, hence HA-MRSA, was 50% (21/42). Spa types t002, t037, t064, t4353 and t12939 were detected and the most frequent were t064 (19%, 8/42) and t037 (12%, 5/42). Conclusion: The MRSA carriage rate in children in eastern Uganda is high (5.7%) and comparable to estimates for Mulago Hospital in Kampala city. Importantly, HA-MRSA (mainly of spa type t037) and CA-MRSA (mainly of spa type t064) coexist in children in the community in eastern Uganda, and due to high proportion of MDR detected, outpatient treatment of MRSA infection in eastern Uganda might be difficult.
700 _aMoses L. Joloba
_923380
700 _a Benon B. Asiimwe
_923721
700 _a Elizeus Rutebemberwa
_923722
700 _aKarin Källander
_923723
700 _a Christine F. Najjuka
_923377
700 _aHannington Baluku
_923724
700 _aFred K. Ashaba
_923725
700 _aBrian Mujuni
_923726
700 _aEdgar Kigozi
_923727
700 _aRaymond Mayanja
_923728
700 _aJeremiah Seni
_919633
700 _a Freddie Bwanga
_923376
856 _uhttps://doi.org/10.1186/s13756-019-0551-1
942 _2ddc
_cVM
999 _c19025
_d19025