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022			_a EISSN 2079-6382
100			_a Vitus Silago _919665
222			_aantimicrobial resistance; extended-spectrum beta-lactamase; Escherichia coli; Klebsiella pneumoniae
245			_aExistence of Multiple ESBL Genes among Phenotypically Confirmed ESBL Producing Klebsiella pneumoniae and Escherichia coli Concurrently Isolated from Clinical, Colonization and Contamination Samples from Neonatal Units at Bugando Medical Center, Mwanza, Tanzania
260			_aMwanza, Tanzania: _bCatholic University of Health and Allied Sciences CUHAS - Bugando & _b MDPI _c21 April 2021
300			_a Pages 476
490			_3 Antibiotics 2021, 10(5), 476 _a Antibiotics 2021, 10(5), 476 _vAntibiotics Volume 10 Issue 5
520			_aAbstract The proportions and similarities of extended-spectrum β-lactamase (ESBL) producing K. pneumoniae (ESBL-KP) and E. coli (ESBL-EC) carrying multiple ESBL genes is poorly known at our setting. This study investigated the existence of multiple ESBL genes (blaCTX-M, blaTEM, and blaSHV) among ESBL-KP and ESBL-EC concurrently isolated from clinical, colonization, and contamination samples from neonatology units in Mwanza-Tanzania. Twenty and 55 presumptive ESBL-EC and ESBL-KP, respectively, from a previous study archived at −80 °C were successfully recovered for this study. Isolates were screened and confirmed for production of ESBLs by phenotypic methods followed by multiplex PCR assay to determine ESBL genes. All (100%) and 97.3% of presumptive ESBL isolates were phenotypically confirmed by Clinical and Laboratory Standards Institute (CLSI) and modified double-disc synergy methods, respectively. About 93.3% (70/75) of phenotypically confirmed ESBL isolates had at least one ESBL gene, whereby for 62.9% (44/70), all ESBL genes (blaCTX-M, blaTEM, and blaSHV) were detected. Eight pairs of ESBL bacteria show similar patterns of antibiotics susceptibility and ESBL genes. ESBL-KP and ESBL-EC, concurrently isolated from clinical, colonization and contamination samples, harbored multiple ESBL genes. Further, eight pairs of ESBL isolates had similar patterns of antibiotics susceptibility and ESBL genes, suggesting transmission of and/or sharing of mobile genetic elements (MGEs) among ESBL-KP and ESBL-EC.
700			_aStephen E. Mshana _915820
700			_aAbubakar S. Hoza _923302
700			_a Athumani M. Lupindu _923301
700			_aKatarina Oravcová _923299
700			_a Louise Matthews _923298
700			_aJeremiah Seni _919633
700			_aHappyness Samson _923622
700			_aDory Kovacs _923297
856			_uhttps://doi.org/10.3390/antibiotics10050476
942			_2ddc _cVM
999			_c19005 _d19005