Molecular Detection of High Risk Human Papillomavirus and Associated Factors among Patient with Esophageal Carcinoma at Bugando Medical Center in Mwanza Tanzania
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TextPublisher number: Wurzburg Road 35, BMC Premises, Post Code: 33102: P. O. Box 1464, Mwanza – Tanzania: Phone: +255 28 298 3384: Fax: +255 28 298 3386: Email: vc@bugando.ac.tz: www.bugando.ac.tzLanguage: English Language: Kiswahili Publication details: Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS – Bugando] : ©2023Description: 41 Pages; Includes ReferencesSubject(s): Summary: Abstract:
Background: Human papillomavirus (HPV) colonization in the esophagus, especially the high-risk subtypes is an increasingly reported risk factor of esophageal carcinoma, however the role of HPV in the development of this condition remains unclear in our setting. Our study aimed to determine the prevalence and associated risk factors of high-risk HPV among (FFPE) tissue blocks with esophageal carcinoma at BMC in Mwanza, Tanzania.
Methodology: DNA samples from 118 FFPE tissue blocks were analyzed by convectional Polymerase chain reaction (PCR) using MY 09/11 degenerate primers targeting the L1 conserved region of HPV genome and specific primers for HPV 16 and HPV 18 targeting the E6/E7 region of types HPV 16 and 18 respectively.
Results: Of the 118 FFPE tissue blocks enrolled, ESCC 107 (91%) was a predominant histological type followed by EAC 11 (9%). Majority belonged to males (68.67%) from Mwanza region (37.29%) with median age of 59.5 (48.5-68) years. 63 (53%) tissue blocks tested positive for high-risk HPV; out of these 43 (68.25%) were from males and 20 (31.75%) were from females. Among the 63 positive high-risks HPV FFPE tissue blocks 41 (65.08%) tested positive for HPV subtype 16, 15 (23.8%) tested positive for HPV subtype 18 and 7 (11.11%) tested positive for other high-risk HPV subtypes. The factors associated with HPV status were alcohol consumption and cigarette smoking (p-values: 0.040 and 0.0010 respectively).
Conclusion: Around half of the esophageal carcinoma FFPE tissue blocks were positive for HPV DNA using PCR majority of which belonged to males. The predominant HPV subtype was HPV 16. The HPV status was significantly associated with cigarette smoking and alcohol use.
| Item type | Current library | Collection | Status | Barcode | |
|---|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | 1 | CUHAS/BM/1000943/T/2 |
Abstract:
Background: Human papillomavirus (HPV) colonization in the esophagus, especially the high-risk subtypes is an increasingly reported risk factor of esophageal carcinoma, however the role of HPV in the development of this condition remains unclear in our setting. Our study aimed to determine the prevalence and associated risk factors of high-risk HPV among (FFPE) tissue blocks with esophageal carcinoma at BMC in Mwanza, Tanzania.
Methodology: DNA samples from 118 FFPE tissue blocks were analyzed by convectional Polymerase chain reaction (PCR) using MY 09/11 degenerate primers targeting the L1 conserved region of HPV genome and specific primers for HPV 16 and HPV 18 targeting the E6/E7 region of types HPV 16 and 18 respectively.
Results: Of the 118 FFPE tissue blocks enrolled, ESCC 107 (91%) was a predominant histological type followed by EAC 11 (9%). Majority belonged to males (68.67%) from Mwanza region (37.29%) with median age of 59.5 (48.5-68) years. 63 (53%) tissue blocks tested positive for high-risk HPV; out of these 43 (68.25%) were from males and 20 (31.75%) were from females. Among the 63 positive high-risks HPV FFPE tissue blocks 41 (65.08%) tested positive for HPV subtype 16, 15 (23.8%) tested positive for HPV subtype 18 and 7 (11.11%) tested positive for other high-risk HPV subtypes. The factors associated with HPV status were alcohol consumption and cigarette smoking (p-values: 0.040 and 0.0010 respectively).
Conclusion: Around half of the esophageal carcinoma FFPE tissue blocks were positive for HPV DNA using PCR majority of which belonged to males. The predominant HPV subtype was HPV 16. The HPV status was significantly associated with cigarette smoking and alcohol use.
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