Comparison study for staining quality and cellularity of 10% neutral buffered formalin over bouins solution in fixing fine needle aspiration samples for cell block techniques at Bugando Medical Centre in Mwanza, Tanzania
Material type:
TextPublisher number: Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz Language: English Language: Kiswahili Publication details: Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS – Bugando] 18.05.2022 Description: 48 Pages; Includes References and AppendicesSubject(s): Summary: Abstract:
Background statement; Cell block (CB) technique refers to the processing of sediments, blood clots or grossly visible fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The CB can be used for ancillary studies like immunohistochemistry and molecular tests. Different fixatives can be used in CB preparation including Bouin’s solution and 10% NBF. Therefore, this study aim to give the similarities and differences in staining quality and cellularity of 10% NBF over Bouin’s solution in fixing Fine Needle Aspiration (FNA) samples for CB technique at BMC.
Objective of the study: To determine the comparison of staining quality and cellularity of 10% NBF over Bouin’s solution in fixing FNA samples for CB technique at BMC.
Methodology: Prospective descriptive study that was conducted from May to June 2022 at CPL in Histopathology unit BMC. Was involving cell block preparation through centrifugation process and microscopic observation for the blocks and convectional smear of minimum 67 samples from FNA biopsies received at BMC CPL. The results were interpreted according to the criteria which are intensity of stain and the histoscore percentages. The results were then be presented by using table forms.
Results: A 70 FNA samples were used in cell block preparation each fixed in both Bouin’s solution and 10% NBF separately. Samples fixed with Bouin’s solution having an average score of 8.4 (60%) while 10% NBF having an average score of 7 (50%) both being within an average range (6-14).Also, sensitivity and specificity of 10% NBF were 65.22% and 66.67% respectively. Prepared normal smears from 70 samples 60 (85.7%) samples were diagnostically suitable while 10 (14.3%) samples were diagnostically unsuitable giving the sensitivity and specificity of 72.4% and 83.3% respectively.
Conclusion: In cytological smears fixed with 10% NBF for cell block preparation about half of the cytological specimen were positive average score. In cytological smear fixed with Bouin’s solution for cell block preparation two third of the cytological specimen were positive average score. Both 10% NBF and Bouin’s solution were within the average score range of histological score. The sensitivity and specificity of convectional normal smear for detection of cytological diagnosis was higher. Therefore, 10% NBF can be appropriate alternative to Bouin’s solution for fixation of FNA samples in cell block preparation at Central Pathology Laboratory, BMC
| Item type | Current library | Collection | Copy number | Status | Barcode | |
|---|---|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | CRECU/2325 | 1 | CRECU/2325 |
Abstract:
Background statement; Cell block (CB) technique refers to the processing of sediments, blood clots or grossly visible fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The CB can be used for ancillary studies like immunohistochemistry and molecular tests. Different fixatives can be used in CB preparation including Bouin’s solution and 10% NBF. Therefore, this study aim to give the similarities and differences in staining quality and cellularity of 10% NBF over Bouin’s solution in fixing Fine Needle Aspiration (FNA) samples for CB technique at BMC.
Objective of the study: To determine the comparison of staining quality and cellularity of 10% NBF over Bouin’s solution in fixing FNA samples for CB technique at BMC.
Methodology: Prospective descriptive study that was conducted from May to June 2022 at CPL in Histopathology unit BMC. Was involving cell block preparation through centrifugation process and microscopic observation for the blocks and convectional smear of minimum 67 samples from FNA biopsies received at BMC CPL. The results were interpreted according to the criteria which are intensity of stain and the histoscore percentages. The results were then be presented by using table forms.
Results: A 70 FNA samples were used in cell block preparation each fixed in both Bouin’s solution and 10% NBF separately. Samples fixed with Bouin’s solution having an average score of 8.4 (60%) while 10% NBF having an average score of 7 (50%) both being within an average range (6-14).Also, sensitivity and specificity of 10% NBF were 65.22% and 66.67% respectively. Prepared normal smears from 70 samples 60 (85.7%) samples were diagnostically suitable while 10 (14.3%) samples were diagnostically unsuitable giving the sensitivity and specificity of 72.4% and 83.3% respectively.
Conclusion: In cytological smears fixed with 10% NBF for cell block preparation about half of the cytological specimen were positive average score. In cytological smear fixed with Bouin’s solution for cell block preparation two third of the cytological specimen were positive average score. Both 10% NBF and Bouin’s solution were within the average score range of histological score. The sensitivity and specificity of convectional normal smear for detection of cytological diagnosis was higher. Therefore, 10% NBF can be appropriate alternative to Bouin’s solution for fixation of FNA samples in cell block preparation at Central Pathology Laboratory, BMC
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