Detecting Plasmodium Sp and Arboviruses, All in One Test
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TextPublisher number: Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz Language: English Series: ; Romanian Archives of Microbiology and Immunology; Bucharest Vol. 80, Iss. 3, (Jul-Sep 2021): 298. Publication details: Mwanza, Tanzania: Romanian Archives of Microbiology and Immunology & Catholic University of Health and Allied Sciences [CUHAS – Bugando] 2021Online resources: Summary: The current study evaluates the ability of a multiplex-reverse transcriptase-polymerase chain reaction test combined with an enzyme linked immunosorbent assay (multiplex-RT-PCR-ELISA) to identify and differentiate nine human mosquitoborne pathogens (eight arboviruses and Plasmodium sp.). Venous blood samples were collected from 132 children (ranging from 5-months to 12-years) who presented with acute high-grade fever (> 38.0 C) and met the WHO criteria of a presumptive malaria infection, to a medical center in Tanzania, between April 2016 and October 2016. Serial dilutions of a standard Zika virus suspension (1x107 viral RNA copies/ml) were added to the master mix containing the 9 primer sets.
| Item type | Current library | Collection | Copy number | Status | Barcode | |
|---|---|---|---|---|---|---|
| RESEARCH ARTICLES | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | RA1043 | -1 | RA1043 |
The current study evaluates the ability of a multiplex-reverse transcriptase-polymerase chain reaction test combined with an enzyme linked immunosorbent assay (multiplex-RT-PCR-ELISA) to identify and differentiate nine human mosquitoborne pathogens (eight arboviruses and Plasmodium sp.). Venous blood samples were collected from 132 children (ranging from 5-months to 12-years) who presented with acute high-grade fever (> 38.0 C) and met the WHO criteria of a presumptive malaria infection, to a medical center in Tanzania, between April 2016 and October 2016. Serial dilutions of a standard Zika virus suspension (1x107 viral RNA copies/ml) were added to the master mix containing the 9 primer sets.
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