Prevalence of Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae Among Humans Working in Abattoirs and Meat Shops in Mwanza City Tanzania.
Material type:
TextPublisher number: Wurzburg Road 35, BMC Premises, Post Code: 33102: P. O. Box 1464, Mwanza – Tanzania: Phone: +255 28 298 3384: Fax: +255 28 298 3386: Email: vc@bugando.ac.tz : www.bugando.ac.tzLanguage: English Language: Kiswahili Publication details: Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS – Bugando] : ©2018Description: ix; 37 Pages; Includes ReferencesSubject(s): Summary: Abstract:
Background: Infections caused by Extended Spectrum Beta-Lactamase-producing Enterobacteriaceae (ESBL-PE) have become of public health concern worldwide. There is limited information on the colonization rate of ESBL-PE among humans working in abattoir and meat shops in the city of Mwanza, Tanzania. This study investigated the prevalence, antibiogram profile and factors associated with ESBL-PE carriage among humans working in abattoir and meat shops in Mwanza city, Tanzania.
Methodology: A cross-sectional study conducted between May and July 2018 among humans working in abattoir and meat in Mwanza city, Tanzania. Their socio-demographics and personal life style related to the study goals were recorded in a data collection tool. Collected stool samples were inoculated onto MacConkey Agar supplemented with 2µg/ml of cefotaxime to screen for ESBL-PE. Positive isolates were presumably confirmed by chromID ESBL (Mac diagnostic). Antimicrobial susceptibility pattern of the bacterial isolates were performed by Kirby-Bauer disk diffusion method as per 2015 clinical laboratory standard institute guidelines. Data were analyzed using STATA version 13 software.
Results: of the 210 human investigated 24 hours humans working in abattoir of which 9 (37.5%) of them carried ESBL-PE and 186 were humans working in meat shops of which 24 (12.9%) of them carried ESBL-PE. A total number of 33 (15.7%) humans working in abattoir and meat shops were colonized with ESBL-PE, whereby one of the 33 ESBL-PE positive humans was colonized with two different ESBL-PE, making the total number of isolates to be 34. Isolated ESBL-PE were Escherichia coli (n=30, 88.24%), Klebsiella pneumoniae (n=3, 8.82%), Salmonella spp (n=1, 2.94). By regarding intermediate zone of inhibition as resistant (I=R), general level of resistance to the antibiotics used for antibiotic susceptibility test were as follows; ceftazidime (97.1%), tetracycline (82.4%), trimethoprim/sulphamethoxazole (100%), Meropenem (0%), gentamycin (29.4%) and ciprofloxacin (32.4%). Animal keeping at home, family size of ≥ 4 people at home, type of toilet used at home (European type or pit latrine), history of admission on past year, antibiotic administration within 4 weeks, water source (Piped or well water) and ages (15-35 years or 36-72 years); used as independent predictors of ESBL-PE carriage but only family size of ≥ 4 people found to be associated with ESBL-PE carriage through this study.
Conclusion: Proportion on ESBL-PE carriage among humans working in Mwanza city abattoir is alarmingly higher compared to humans working in meet shops. Education on AMR cross-transmission and advocacy for hygienic practices among humans working in abattoir and meat together with their working environment should be emphasized.
| Item type | Current library | Collection | Status | Barcode | |
|---|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | 1 | UD0307 |
Abstract:
Background: Infections caused by Extended Spectrum Beta-Lactamase-producing Enterobacteriaceae (ESBL-PE) have become of public health concern worldwide. There is limited information on the colonization rate of ESBL-PE among humans working in abattoir and meat shops in the city of Mwanza, Tanzania. This study investigated the prevalence, antibiogram profile and factors associated with ESBL-PE carriage among humans working in abattoir and meat shops in Mwanza city, Tanzania.
Methodology: A cross-sectional study conducted between May and July 2018 among humans working in abattoir and meat in Mwanza city, Tanzania. Their socio-demographics and personal life style related to the study goals were recorded in a data collection tool. Collected stool samples were inoculated onto MacConkey Agar supplemented with 2µg/ml of cefotaxime to screen for ESBL-PE. Positive isolates were presumably confirmed by chromID ESBL (Mac diagnostic). Antimicrobial susceptibility pattern of the bacterial isolates were performed by Kirby-Bauer disk diffusion method as per 2015 clinical laboratory standard institute guidelines. Data were analyzed using STATA version 13 software.
Results: of the 210 human investigated 24 hours humans working in abattoir of which 9 (37.5%) of them carried ESBL-PE and 186 were humans working in meat shops of which 24 (12.9%) of them carried ESBL-PE. A total number of 33 (15.7%) humans working in abattoir and meat shops were colonized with ESBL-PE, whereby one of the 33 ESBL-PE positive humans was colonized with two different ESBL-PE, making the total number of isolates to be 34. Isolated ESBL-PE were Escherichia coli (n=30, 88.24%), Klebsiella pneumoniae (n=3, 8.82%), Salmonella spp (n=1, 2.94). By regarding intermediate zone of inhibition as resistant (I=R), general level of resistance to the antibiotics used for antibiotic susceptibility test were as follows; ceftazidime (97.1%), tetracycline (82.4%), trimethoprim/sulphamethoxazole (100%), Meropenem (0%), gentamycin (29.4%) and ciprofloxacin (32.4%). Animal keeping at home, family size of ≥ 4 people at home, type of toilet used at home (European type or pit latrine), history of admission on past year, antibiotic administration within 4 weeks, water source (Piped or well water) and ages (15-35 years or 36-72 years); used as independent predictors of ESBL-PE carriage but only family size of ≥ 4 people found to be associated with ESBL-PE carriage through this study.
Conclusion: Proportion on ESBL-PE carriage among humans working in Mwanza city abattoir is alarmingly higher compared to humans working in meet shops. Education on AMR cross-transmission and advocacy for hygienic practices among humans working in abattoir and meat together with their working environment should be emphasized.
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