The Magnitude of Bla CTX-M3G Among Extended Spectrum Beta-Lactamase Producing Gram Negative Bacteria Isolated From Culture in Mwanza Tanzania
Material type:
TextPublication details: Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS – Bugando] © Phone: +255 28 298 3384 : Fax: +255 28 298 3386 : Email: vc@bugando.ac.tz : Website: www.bugando.ac.tz: ©2018Description: ii; 28 Pages; includes references and appendincesSubject(s): Summary: Abstract:
Background: Multi-drug resistant organisms (MDRO) are known to cause serious pathophysiology among humans. There is limited information on MDRO causing bacteriospermia among presumptive infertile men in Mwanza, Tanzania. The current study aims to determine the prevalence of MDRO causing bacterospemia among presumptive infertile men in Mwanza Tanzania.
Methods: it was laboratory experimental based study, conducted during July 2018 involving 38 presumptive infertile men. Minus 40 degree Celsius archived gram negative bacteria isolated from semen culture where analyzed at CUHAS MPL and NIMR, gram negative bacteria isolated recovered by sub-culturing on MacConkey agar (MCA) plates. ESBL producing GNB was screened by using MCA-c and confirmed by double disc synergy and molecular characterized to determine carriage of blaCTX-M genes. STATA version 13.0 was used for data analysis.
Results: A total of 38 participants were involved in the study had presented with bacteriospermia of which the most isolated bacteria was GNB. Among 38 gram negative isolated bacteria, ESBL Producer were 12/38(31.6%), and the most isolated bacterial species was K. pneomoniae 7/12 (58.3%)and out of 12 phenotypic ESBL producers, 9/12 (75% ) were carrying ESBL blaCTX-M gene.
Conclusion: there is high prevalence of ESBL producing gram negative bacteria causing bacteriospermia among presumptive infertile men in Mwanza – Tanzania, whose drug of choice was meropenem and ciprofloxacin.
| Item type | Current library | Collection | Status | Barcode | |
|---|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | 1 | UD0104 |
Abstract:
Background: Multi-drug resistant organisms (MDRO) are known to cause serious pathophysiology among humans. There is limited information on MDRO causing bacteriospermia among presumptive infertile men in Mwanza, Tanzania. The current study aims to determine the prevalence of MDRO causing bacterospemia among presumptive infertile men in Mwanza Tanzania.
Methods: it was laboratory experimental based study, conducted during July 2018 involving 38 presumptive infertile men. Minus 40 degree Celsius archived gram negative bacteria isolated from semen culture where analyzed at CUHAS MPL and NIMR, gram negative bacteria isolated recovered by sub-culturing on MacConkey agar (MCA) plates. ESBL producing GNB was screened by using MCA-c and confirmed by double disc synergy and molecular characterized to determine carriage of blaCTX-M genes. STATA version 13.0 was used for data analysis.
Results: A total of 38 participants were involved in the study had presented with bacteriospermia of which the most isolated bacteria was GNB. Among 38 gram negative isolated bacteria, ESBL Producer were 12/38(31.6%), and the most isolated bacterial species was K. pneomoniae 7/12 (58.3%)and out of 12 phenotypic ESBL producers, 9/12 (75% ) were carrying ESBL blaCTX-M gene.
Conclusion: there is high prevalence of ESBL producing gram negative bacteria causing bacteriospermia among presumptive infertile men in Mwanza – Tanzania, whose drug of choice was meropenem and ciprofloxacin.
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