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The role of uncentrifuged urine microscopy in the diagnosis of urinary tract infection

By: Contributor(s): Material type: TextTextPublisher number: Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz Language: English Series: ; Tanzan Med J Volume 25 Issue 1 Publication details: Mwanza, Tanzania: Tanzan Med J & Catholic University of Health and Allied Sciences [CUHAS – Bugando] 2011/1/1Description: Pages 17-18Online resources: Summary: Background: Urinary tract infection (UTI) is one of the most common nosocomial bacterial infections prevalent in both males and females. UTI is diagnosed on the basis of clinical symptoms, microscopy and culture of urine. This study was done to establish the role of the routine uncentrifuged urine microscopy using culture as gold standard at Bugando Medical Centre. Methods: A total of 389 urine specimens were processed. Urine microscopy was done to all specimens to determine WBC/high power field. All specimens were cultured on Mackonkey, Blood agar and CLED. The presence of 5 or more WBC/HPF was considered as positive and presence of 105CFU/ml was reported as significant bacteriuria. Urine microscopy results and culture results were statistically compared using p≤ 0.05 as significant. Results: The prevalence of UTI by culture and urine microscopy was 38.8% and 13.3% respectively. Using culture as gold standard the sensitivity, specificity and positive predictive value of uncentrifuged urine were 34%, 84% and 58% respectively. There was significant association between presence of≥ 5 WBC/HPF and positive urine
Item type: RESEARCH ARTICLES
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Item type Current library Collection Copy number Status
RESEARCH ARTICLES MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO NFIC RA0826 -1
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Background: Urinary tract infection (UTI) is one of the most common nosocomial bacterial infections prevalent in both males and females. UTI is diagnosed on the basis of clinical symptoms, microscopy and culture of urine. This study was done to establish the role of the routine uncentrifuged urine microscopy using culture as gold standard at Bugando Medical Centre.

Methods: A total of 389 urine specimens were processed. Urine microscopy was done to all specimens to determine WBC/high power field. All specimens were cultured on Mackonkey, Blood agar and CLED. The presence of 5 or more WBC/HPF was considered as positive and presence of 105CFU/ml was reported as significant bacteriuria. Urine microscopy results and culture results were statistically compared using p≤ 0.05 as significant.

Results: The prevalence of UTI by culture and urine microscopy was 38.8% and 13.3% respectively. Using culture as gold standard the sensitivity, specificity and positive predictive value of uncentrifuged urine were 34%, 84% and 58% respectively. There was significant association between presence of≥ 5 WBC/HPF and positive urine

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