Characterization of Genes Encoding for Amp-C Beta-Lactamase Production among Beta-Lactamase Producing Gram-Negative Bacteria Isolated from Inanimate Surfaces of Magu, Sumve, and Misungwi District Hospitals in Mwanza, Tanzania.
- Mwanza, Tanzania | Catholic University of Health and Allied Sciences [CUHAS-Bugando] | 2024.
- 40 Pages Includes References
Abstract:
Background: Hospital inanimate surfaces can serve as possible external reservoirs for the spread of Gram-negative bacteria that produce beta lactamases. Previous studies have documented on the prevalence of hospital surfaces contaminated with ESBL and carbapenemase producing GNB at various tertiary, regional and district hospitals. However, little is documented on the presence of Amp-C producing GNB in these hospitals. Therefore, this study determined the proportion and predominant GNB species producing Amp-C beta-lactamases among Ceph-R-GNB isolated from inanimate hospital surfaces at Magu, Sumve, and Misungwi district hospitals in Mwanza, Tanzania.
Methods: A cross-sectional laboratory-based study was conducted between May and August 2024 using achieved isolated from Magu, Sumve and Misungwi district hospitals in Mwanza, Tanzania. Isolates were recovered using MCA-C followed by screening for Amp-C producing GNB using cefoxitin disk (30µg) and confirmed by DDST using Cefoxitin (30µg), ceftazidime (30 μg) and cefotaxime (30 μg) discs and interpreted using CLSI guideline 2020. Data was then entered in Microsoft excel and analyzed by using STATA version 15.0.
Results: A total of 299 bacteria species were recovered, of which 61.5% (184/299) were detected as Amp-C beta-lactamase producing GNB presumptive (cefoxitin resistant). Additionally, 6.02% (18/299) were Amp-C positive phenotypes. Predominantly, Amp-C positive isolates were isolated from Magu district hospital, 94.4% (17/18) and mostly in Klebsiella oxytoca 27.8% (5/18) followed by Enterobacter aerogenes 22.2% (4/18) and Escherichia coli 19.2% (3/18).
Conclusion: There is existence of Amp-C beta-lactamase producing GNB contaminating inanimate hospital surfaces mostly at Magu district hospital in Mwanza, Tanzania, commonly found in Klebsiella oxytoca, Enterobacter aerogenes and Escherichia coli. Therefore, proper infection prevention and control measures are to be enhanced.
Wurzburg Road 35, Premises, Post Code: 33102 | P. O. Box 1464 Mwanza, Tanzania | Phone: (255) 28-298-3384 | Fax: (255) 28-298-3386 | Email: vc@bugando.ac.tz | Website: www.bugando.ac.tz