TY - BOOK AU - P Koliopoulos AU - NM Kayange AU - T Daniel AU - F Huth AU - B Gröndahl AU - GC Medina-Montaño TI - Detecting Plasmodium Sp and Arboviruses, All in One Test PY - 2021/// CY - Mwanza, Tanzania: PB - Romanian Archives of Microbiology and Immunology &, Catholic University of Health and Allied Sciences [CUHAS – Bugando] N2 - The current study evaluates the ability of a multiplex-reverse transcriptase-polymerase chain reaction test combined with an enzyme linked immunosorbent assay (multiplex-RT-PCR-ELISA) to identify and differentiate nine human mosquitoborne pathogens (eight arboviruses and Plasmodium sp.). Venous blood samples were collected from 132 children (ranging from 5-months to 12-years) who presented with acute high-grade fever (> 38.0 C) and met the WHO criteria of a presumptive malaria infection, to a medical center in Tanzania, between April 2016 and October 2016. Serial dilutions of a standard Zika virus suspension (1x107 viral RNA copies/ml) were added to the master mix containing the 9 primer sets UR - https://www.proquest.com/openview/0465855cfcc19d7123df819006c8bb43/1?pq-origsite=gscholar&cbl=4585458 ER -