TY - BOOK AU - Vivanco,Maria del Mar TI - Mammary stem cells: methods and protocols T2 - Methods in molecular biology, SN - 9781493925186 (hbk : alk. paper) AV - QH588.S83 M36 2015 U1 - 616.02/774 23 PY - 2015///] CY - New York PB - Humana Press KW - Stem cells KW - Laboratory manuals KW - Mammary glands KW - Molecular aspects KW - Breast KW - Cytology KW - Mammary Glands, Animal KW - cytology KW - Laboratory Manuals KW - Stem Cells KW - Mice N1 - Includes bibliographical references and index; Breast cancer stem cells: current advances and clinical implications; Ming Luo [and six others] --; A protocol for studying embryonic mammary progenitor cells during mouse mammary primordial development in explant culture; Naoko Kogata and Beatrice A. Howard --; FACS sorting mammary stem cells; Oihana Iriondo, Miriam Rábano, and María d.M. Vivanco --; Side population; Fariba Behbod and María d.M. Vivanco --; Single-cell genome and transcriptome processing prior to high-throughput sequencing; Ana M. Aransay [and three others] --; SHotgun proteomocs on tissue specimens extracted with acid guanidinium-thiocyanate-phenol-chloroform; René B. H. Braakman, Anieta M. Sieuwerts, and Arzu Umar --; Antibody-based capture of target peptides in multiple reaction monitoring experiments; Tommaso De Marchi [and three others] --; Lentiviral transduction of mammary epithelial cells; Richard Iggo and Elodie Richard --; The transplantation of mouse mammary epithelial cells into cleared mammary fat pads; Marisa M Faraldo, Marina A. Glukhova, and Marie-Ange Deugnier --; Humanization of the mouse mammary gland; A. Wronski, L. M. Arendt, and Charlotte Kuperwasser --; Lineage tracing in the mammary gland using Cre/lox technology and fluorescent reporter alleles; Renée van Amerongen --; Modeling the breast cancer bone metastatic niche in complex three-dimensional cocultures; Rebecca Marlow and Gabriela Dontu --; Mammary cancer stem cells reinitiation assessment at the metastatic niche: the lung and bone; Marc Guiu [and four others] --; Nanomechanical characterization of living mammary tissues by atomic force microscopy; Marija Plodinec and Anna Marciniak-Czochra --; Mathematical modeling as a tool to understand cell self-renewal and differentiation; Philipp Getto and Anna Marciniak-Czochra --; Mammary stem cells: a clinician's view; José Schneider ER -