Comparison of Modified Ultrafast Papanicolaou Staining Technique with Conventional Papanicolaou Staining Technique in Assessment of Cytological Samples at Bugando Medical Center in Mwanza, Tanzania.
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TextPublisher number: Wurzburg Road 35, Premises, Post Code: 33102 | P. O. Box 1464 Mwanza, Tanzania | Phone: (255) 28-298-3384 | Fax: (255) 28-298-3386 | Email: vc@bugando.ac.tz | Website: www.bugando.ac.tz Language: English Language: Kiswahili Publication details: Mwanza, Tanzania | Catholic University of Health and Allied Sciences [CUHAS-Bugando] | 2024.Description: 40 Pages; Includes ReferencesSubject(s): Summary: Abstract:
Background: Cytological analysis plays a significant role in the early detection and diagnosis of several diseases, including cancer. The Papanicolaou (PAP) stain was developed by Dr. George N Papanicolaou, has long been considered the gold standard for screening and diagnosing cervical cancer and other cytological abnormalities. This method has some limitations including labor-intensive and time-consuming. To overcome these challenges modified ultrafast Pap staining methods have been developed.
Objective: To compare the staining quality of the Modified Ultrafast Papanicolaou with Conventional Papanicolaou Staining techniques in cytological samples at BMC.
Methods: This was a cross-sectional laboratory-based study conducted from July to August 2024 at BMC at Histopathology & morbid anatomy laboratory, convenience sampling technique was used to obtain cytological samples. Two smears were prepared for conventional PAP stain and MUFP stain technique, microscopic examination was done to compare the staining quality of the two techniques. Data was inserted into excel for cleaning and analyzed using STATA version 15, where Percentage or fraction were be used to summarize categorical variables while mean (STD)/median (IQR) was used for continuous variables. Ch2/Fisher’s exact was used to show association between parameters in conventional PAP stain and MUFP stain. P-value of <0.05 was considered as statistically significant.
Result: Out of 82 cytological samples, clean background stain was seen in 100% of smears stained by MUFP and hemorrhagic background was seen in 24.4% of PAP smears, majority of PAP smears 91.5% had crisp nucleus and 12.2% of MUFP has dull nucleus, 74.4% of MUFP smears showed well preserved cellular morphology, 53.7% of PAP smears had moderate preservation and 8.5% of MUFP smears showed no preservation of cellular morphology.
Conclusion: MUFP Stain shows a clear background & crisp nuclear features when compared to conventional PAP stain. Also, the preservation of cellular morphology and overall staining in MUFP is almost equal to that of conventional PAP stain 
| Item type | Current library | Status | Barcode | |
|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | Not for loan | 20240918111155.0 |
Abstract:
Background: Cytological analysis plays a significant role in the early detection and diagnosis of several diseases, including cancer. The Papanicolaou (PAP) stain was developed by Dr. George N Papanicolaou, has long been considered the gold standard for screening and diagnosing cervical cancer and other cytological abnormalities. This method has some limitations including labor-intensive and time-consuming. To overcome these challenges modified ultrafast Pap staining methods have been developed.
Objective: To compare the staining quality of the Modified Ultrafast Papanicolaou with Conventional Papanicolaou Staining techniques in cytological samples at BMC.
Methods: This was a cross-sectional laboratory-based study conducted from July to August 2024 at BMC at Histopathology & morbid anatomy laboratory, convenience sampling technique was used to obtain cytological samples. Two smears were prepared for conventional PAP stain and MUFP stain technique, microscopic examination was done to compare the staining quality of the two techniques. Data was inserted into excel for cleaning and analyzed using STATA version 15, where Percentage or fraction were be used to summarize categorical variables while mean (STD)/median (IQR) was used for continuous variables. Ch2/Fisher’s exact was used to show association between parameters in conventional PAP stain and MUFP stain. P-value of <0.05 was considered as statistically significant.
Result: Out of 82 cytological samples, clean background stain was seen in 100% of smears stained by MUFP and hemorrhagic background was seen in 24.4% of PAP smears, majority of PAP smears 91.5% had crisp nucleus and 12.2% of MUFP has dull nucleus, 74.4% of MUFP smears showed well preserved cellular morphology, 53.7% of PAP smears had moderate preservation and 8.5% of MUFP smears showed no preservation of cellular morphology.
Conclusion: MUFP Stain shows a clear background & crisp nuclear features when compared to conventional PAP stain. Also, the preservation of cellular morphology and overall staining in MUFP is almost equal to that of conventional PAP stain 
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