A Comperative Study to Examine the Effectiveness on Bluing Between Tap Water, Scott’s Tap Water and Ammonium Hydroxide in Hematoxylin and Eosin Staining Procedure at Bugando Medical Centre.
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TextPublisher number: Wurzburg Road 35, Premises, Post Code: 33102 | P. O. Box 1464 Mwanza, Tanzania | Phone: (255) 28-298-3384 | Fax: (255) 28-298-3386 | Email: vc@bugando.ac.tz | Website: www.bugando.ac.tz Language: English Language: Kiswahili Publication details: Mwanza, Tanzania | Catholic University of Health and Allied Sciences [CUHAS-Bugando] | 2024. Description: 34 Pages; Includes ReferencesSubject(s): Summary: Abstract:
Background: Bluing is the process which changes the initial soluble reddish-purple Hematoxylin into an insoluble darker bluish-purple. Commonly used bluing agents in histopathology are tap water, Scott’s tap water and Ammonium Hydroxide water. The aim of this study is to assess the efficacy of Scotts tape water and 0.05% Ammonium hydroxide water as an alternative to tap water as bluing agents. Considering the introduction of automated staining machines which does not requires the use tap water as a bluing agent due to fluctuations in its pH, or may contain contaminants that can distain hematoxylin, so this comparative study can change the staining protocol at BMC-histopathology laboratory of using tape water as bluing agent to Scott’s tap water or 0.05% ammonium hydroxide solution, thereby enhancing diagnostic accuracy and reliability.
Objectives: This study intended to determine the comparative effectiveness of three different bluing agents which are tape water, Scott’s tape water and 0.05% ammonium hydroxide solution.
Methodologies: This comparative laboratory-based study was conducted to tissue samples obtained from human tissue biopsies from May to August 2024. Specimens that will meet standards will be fixed, processed, embedded, sectioned, stained using tape water, Scott’s tape water and 0.05% ammonium hydroxide solution as bluing agents and observed microscopically accessing nuclear intensity, Nuclear Morphology, Cytoplasmic Intensity, Bluing and Clarity.
Results: The scoring criteria included nuclear intensity, Nuclear Morphology, Cytoplasmic Intensity, Bluing and Clarity which were scored as Excellent, Good, Satisfactory and Poor. Scott’s tape water showed excellent performance with 85% in Nuclear intensity,96% in Nuclear morphology,7% in Cytoplasmic intensity,88 % in bluing and 85% in clarity. Tape water followed with the following scores, 73% in Nuclear intensity,94% % in Nuclear morphology,72% in Cytoplasmic intensity,73% in bluing and 73.5% in clarity. Then followed by ammonium solution where 64% in Nuclear intensity,91% in Nuclear morphology,70% in Cytoplasmic intensity,64.5% in bluing and 63% in clarity.
Conclusion: From the results obtained Scott’s tape water can be used as bluing agent to replace tape water. Thus, ammonium solution cannot be used as replacement to tape water since it performed poor in terms of bluing, Nuclear intensity and overall clarity.
| Item type | Current library | Status | Barcode | |
|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | Not for loan | 20240918083016.0 |
Abstract:
Background: Bluing is the process which changes the initial soluble reddish-purple Hematoxylin into an insoluble darker bluish-purple. Commonly used bluing agents in histopathology are tap water, Scott’s tap water and Ammonium Hydroxide water. The aim of this study is to assess the efficacy of Scotts tape water and 0.05% Ammonium hydroxide water as an alternative to tap water as bluing agents. Considering the introduction of automated staining machines which does not requires the use tap water as a bluing agent due to fluctuations in its pH, or may contain contaminants that can distain hematoxylin, so this comparative study can change the staining protocol at BMC-histopathology laboratory of using tape water as bluing agent to Scott’s tap water or 0.05% ammonium hydroxide solution, thereby enhancing diagnostic accuracy and reliability.
Objectives: This study intended to determine the comparative effectiveness of three different bluing agents which are tape water, Scott’s tape water and 0.05% ammonium hydroxide solution.
Methodologies: This comparative laboratory-based study was conducted to tissue samples obtained from human tissue biopsies from May to August 2024. Specimens that will meet standards will be fixed, processed, embedded, sectioned, stained using tape water, Scott’s tape water and 0.05% ammonium hydroxide solution as bluing agents and observed microscopically accessing nuclear intensity, Nuclear Morphology, Cytoplasmic Intensity, Bluing and Clarity.
Results: The scoring criteria included nuclear intensity, Nuclear Morphology, Cytoplasmic Intensity, Bluing and Clarity which were scored as Excellent, Good, Satisfactory and Poor. Scott’s tape water showed excellent performance with 85% in Nuclear intensity,96% in Nuclear morphology,7% in Cytoplasmic intensity,88 % in bluing and 85% in clarity. Tape water followed with the following scores, 73% in Nuclear intensity,94% % in Nuclear morphology,72% in Cytoplasmic intensity,73% in bluing and 73.5% in clarity. Then followed by ammonium solution where 64% in Nuclear intensity,91% in Nuclear morphology,70% in Cytoplasmic intensity,64.5% in bluing and 63% in clarity.
Conclusion: From the results obtained Scott’s tape water can be used as bluing agent to replace tape water. Thus, ammonium solution cannot be used as replacement to tape water since it performed poor in terms of bluing, Nuclear intensity and overall clarity.
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