Quality Index of Modified ultrafast Giemsa staining in a cytological smear of Fine Needle Aspirations at Bugando Medical Center, Mwanza Tanzania.
Material type:
TextPublisher number: Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz Language: English Language: Kiswahili Publication details: Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS – Bugando] : ©2023Description: 26 Pages; Includes ReferencesSubject(s): Summary: Abstract:
Introduction: Given the current trend of quick turnaround times for laboratory sample reporting, it is believed that the initial interpretation of FNA specimens should be done right away. MUFG is mostly favored because it is only a single-step stain, much cheaper, quicker, and with comparable transparency; thus, it can be used in quick assessment that complements the traditional Papanicolaou stain and has advantages in demonstrating specific cytologic features of benign and malignant lesions. The study aimed at addressing the research gap by evaluating the quality index of MUFG-stained FNA smears in comparison to Pap-stained smears in Tanzania.
Methodology: The study was a comparative prospective study conducted at Bugando Medical Center Pathology Laboratory. The study population were all patients presenting for FNAC at the hospital. Data was collected from 81 FNAC samples, where staining processes were done by lab scientist and interpretation done by a pathologist.
Results: From the study the overall staining quality was assessed between the smears there as a slight difference on the stain having an excellent staining quality with PAP and MUFG having 23.57% and 27.16 respectively. On the assessment of Quality Index for all the slides for each organ. MUFG had the highest QI among majority tissue sample sites collected (3/4) with the highest being in the axillary lymph nodes samples with a mean QI of (0.96). The study showed there was an association between the stain used and nuclear features observed with a p-value of 0.001 for fair nucleus and 0.002 for good nuclear character observed in MUFG stain.
Conclusion: The study highlights that the MUFG having an overall upper hand over the use of the conventional PAP smear in staining of various FNAC samples. However, the difference is minor when multiples parameters are kept into comparison thus reducing the urgency of shifting to the MUFG from PAP and using MUFG as a conventional day to day stain. More studies should be done to highlight other factors such as cost, time used, that affect the quality index of the stains and overall staining qualities.
Keywords: Quality Index, Modified Ultrafast Giemsa Stain, Papanicolaou stain.
| Item type | Current library | Collection | Status | Barcode | |
|---|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | 1 | CUHAS/BM/1000952/T/2 |
Abstract:
Introduction: Given the current trend of quick turnaround times for laboratory sample reporting, it is believed that the initial interpretation of FNA specimens should be done right away. MUFG is mostly favored because it is only a single-step stain, much cheaper, quicker, and with comparable transparency; thus, it can be used in quick assessment that complements the traditional Papanicolaou stain and has advantages in demonstrating specific cytologic features of benign and malignant lesions. The study aimed at addressing the research gap by evaluating the quality index of MUFG-stained FNA smears in comparison to Pap-stained smears in Tanzania.
Methodology: The study was a comparative prospective study conducted at Bugando Medical Center Pathology Laboratory. The study population were all patients presenting for FNAC at the hospital. Data was collected from 81 FNAC samples, where staining processes were done by lab scientist and interpretation done by a pathologist.
Results: From the study the overall staining quality was assessed between the smears there as a slight difference on the stain having an excellent staining quality with PAP and MUFG having 23.57% and 27.16 respectively. On the assessment of Quality Index for all the slides for each organ. MUFG had the highest QI among majority tissue sample sites collected (3/4) with the highest being in the axillary lymph nodes samples with a mean QI of (0.96). The study showed there was an association between the stain used and nuclear features observed with a p-value of 0.001 for fair nucleus and 0.002 for good nuclear character observed in MUFG stain.
Conclusion: The study highlights that the MUFG having an overall upper hand over the use of the conventional PAP smear in staining of various FNAC samples. However, the difference is minor when multiples parameters are kept into comparison thus reducing the urgency of shifting to the MUFG from PAP and using MUFG as a conventional day to day stain. More studies should be done to highlight other factors such as cost, time used, that affect the quality index of the stains and overall staining qualities.
Keywords: Quality Index, Modified Ultrafast Giemsa Stain, Papanicolaou stain.
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