Susceptibility of Acinetobacter species to antiseptic agents by microtiter assay technique at Bugando Medical Centre, Mwanza, Tanzania
Material type:
TextPublisher number: Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz Language: English Language: Kiswahili Publication details: Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS – Bugando] ©22.08.2022 Description: x; 25 Pages; Includes References and AppendicesSubject(s): Summary: Abstract:
Background: The success of preventing surgical site infection is threatened by bacteria that are resistant to antiseptic agents used for preoperative skin preparation. Data concerning other Gram negative rods such as Acinetobacter spp. is not known, moreover performance of microtiter assay technique is not documented at BMC, Mwanza-Tanzania. This study determined susceptibility of Acinetobacter spp. to antiseptic agents by microtiter assay technique at Bugando Medical Centre, Mwanza-Tanzania.
Method: This was a cross-sectional laboratory based study conducted from April to May 2022. A total of 173 Acinetobacter spp. which are archived at -80°C in Microbiology Laboratory at CUHAS were recovered by subculture on MacConkey agar (MCA) plates then incubated in ambient air at 37°C for 24 hours. Microtiter assay technique was used to test the susceptibility of Acinetobacter species towards 70% methylated spirit, 6% hydrogen peroxide and 10% povidone iodine at 1 minute, 5 minutes and 10 minutes contact times. STATA software version 15 was used for data analysis.
Results: A total of 64 Acinetobacter spp. whereby 31 (48.4%) isolates obtained from hospital environment, 17 (26.6%) isolates obtained rectal carriage, and 16 (25.0%) isolates obtained from clinical sample were used in this study. One quarter (25.0%) of the bacteria isolates were non susceptible to antiseptics used in preoperative skin preparations. However none exhibited resistance to 10% povidone iodine.
Conclusion: The magnitude of resistance was high at 1 minute contact time compared to 5 minutes and 10 minutes contact times hence the time of exposure was paramount in efficacy of antiseptics.
| Item type | Current library | Collection | Copy number | Status | Barcode | |
|---|---|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | CRECU/2291 | 1 | CRECU/2291 |
Abstract:
Background: The success of preventing surgical site infection is threatened by bacteria that are resistant to antiseptic agents used for preoperative skin preparation. Data concerning other Gram negative rods such as Acinetobacter spp. is not known, moreover performance of microtiter assay technique is not documented at BMC, Mwanza-Tanzania. This study determined susceptibility of Acinetobacter spp. to antiseptic agents by microtiter assay technique at Bugando Medical Centre, Mwanza-Tanzania.
Method: This was a cross-sectional laboratory based study conducted from April to May 2022. A total of 173 Acinetobacter spp. which are archived at -80°C in Microbiology Laboratory at CUHAS were recovered by subculture on MacConkey agar (MCA) plates then incubated in ambient air at 37°C for 24 hours. Microtiter assay technique was used to test the susceptibility of Acinetobacter species towards 70% methylated spirit, 6% hydrogen peroxide and 10% povidone iodine at 1 minute, 5 minutes and 10 minutes contact times. STATA software version 15 was used for data analysis.
Results: A total of 64 Acinetobacter spp. whereby 31 (48.4%) isolates obtained from hospital environment, 17 (26.6%) isolates obtained rectal carriage, and 16 (25.0%) isolates obtained from clinical sample were used in this study. One quarter (25.0%) of the bacteria isolates were non susceptible to antiseptics used in preoperative skin preparations. However none exhibited resistance to 10% povidone iodine.
Conclusion: The magnitude of resistance was high at 1 minute contact time compared to 5 minutes and 10 minutes contact times hence the time of exposure was paramount in efficacy of antiseptics.
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