Molecular types of Candida Species from Clinical Specimens at BMC in Mwanza City, Tanzania.
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TextPublication details: Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS – Bugando] Phone: +255 28 298 3384 : Fax: +255 28 298 3386 : Email: vc@bugando.ac.tz : Website: www.bugando.ac.tz : ©2018Description: vii; 23 Pages; Includes Refferences and AppendicesSubject(s): Summary: Abstract:
Introduction: Different genotypes of Candida albicans are implicated in causing different types of infections with some being more virulent than others. This study was done to determine genotypes of candida albicans and associate them with clinical infection they cause.
Objectives: The aim of this is to characterize archived clinical isolates of Candida albicans from patients in Mwanza, Tanzania.
Methods: Isolates of C. albicans were characterized using PCR-RAPD. DNA was extracted and then PCR performed using primer, SMI (5’ –GGTGCGGGA-3’). Data were analyzed using STATA version 13 software following the objectives of the study.
Results: Of 71 C. albicans, a total of 73.2% (52/71) were successfully amplified. Using SM 1 primer, a total of 82.6% (43/52) clusters of genetic population differences was observed. All the isolates had about 45% genetic similarities in three groups (I, II, and III). A total of 3 main subgroups were obtained nominated as A, B and C, The major group was I which contain 30/52 (57.6%) isolates with 50% genetic population similarity.
Conclusion: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (Blood of Diabetic patients and gastritis). Further studies to investigate the source of nosocomial infections using RAPD are recommended.
| Item type | Current library | Collection | Status | Barcode | |
|---|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | 1 | UD1330 |
Abstract:
Introduction: Different genotypes of Candida albicans are implicated in causing different types of infections with some being more virulent than others. This study was done to determine genotypes of candida albicans and associate them with clinical infection they cause.
Objectives: The aim of this is to characterize archived clinical isolates of Candida albicans from patients in Mwanza, Tanzania.
Methods: Isolates of C. albicans were characterized using PCR-RAPD. DNA was extracted and then PCR performed using primer, SMI (5’ –GGTGCGGGA-3’). Data were analyzed using STATA version 13 software following the objectives of the study.
Results: Of 71 C. albicans, a total of 73.2% (52/71) were successfully amplified. Using SM 1 primer, a total of 82.6% (43/52) clusters of genetic population differences was observed. All the isolates had about 45% genetic similarities in three groups (I, II, and III). A total of 3 main subgroups were obtained nominated as A, B and C, The major group was I which contain 30/52 (57.6%) isolates with 50% genetic population similarity.
Conclusion: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (Blood of Diabetic patients and gastritis). Further studies to investigate the source of nosocomial infections using RAPD are recommended.
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