In Vitro Antibacterial Activity of Azadirachta Indica (Neem Tree) Against Esbl-Producing Escherichia Coli Uropa Thogenic Clinical Isolates at Bugando Medical Centre, Mwanza
Material type:
TextPublisher number: Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz Language: English Language: Kiswahili Publication details: Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS - Bugando] : ©31.08.2018Description: xi; 29 Pages; Includes References and AppendicesSubject(s): Summary: Abstract:
Background: Antimicrobial resistance is a global treat which requires immediate action in searching for alternative antimicrobial therapeutics options. Azadirachta indica is the plant with multiple antimicrobial affects against bacterial, protozoa and fungus, and therefore offers a potential alternative option.
Objective: To determine anti-bacterial activities of A. indica leaf extract against ESBL producing uropathogenic Escherichia coli (UPEC) clinical isolates at Bugando medical centre in Mwanza.
Material and methods: The 70% methanol and distilled water A. indica leaf extracts were prepared. Ten UPEC clinical isolates were cultured into Muller Hinton agar with A. indica impregnated disks and other conventional antimicrobial disks. Zone of inhibition and minimum bacterial concentration (MBC) were obtained by using disc diffusion method and broth microdilution methods, respectively.
Results: The A. indica leaf 70% methanol extract showed antibacterial activity against 30% of ESBL producing UPEC at a concentration of 0.3g/ml. The MBC was at 0.6g/ml concentration. The diameter of inhibition of A. indica to 10 UPEC isolates ranged from 8 mm to 20 mm. The overall percentage of resistance of 10 UPEC isolates for other antibiotics were: meropenem (0%), gentamicin (70%), ciprofloxacin (90%), ceftazidime (100%) and trimethoprim sulfamethoxazole (100%).
Conclusion and recommendations: The A. indica leaf extract exhibited the anti-bacterial activity against 30% of ESBL producing E. coli at a concentration of 0.3g/ml. This antimicrobial effect was shown only 70% methanol extract and not with aqueous extract. Future to assess active ingredients and toxicological profile of A. indica extracts are recommended.
| Item type | Current library | Collection | Status | Barcode | |
|---|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | 1 | UD0113 |
Abstract:
Background: Antimicrobial resistance is a global treat which requires immediate action in searching for alternative antimicrobial therapeutics options. Azadirachta indica is the plant with multiple antimicrobial affects against bacterial, protozoa and fungus, and therefore offers a potential alternative option.
Objective: To determine anti-bacterial activities of A. indica leaf extract against ESBL producing uropathogenic Escherichia coli (UPEC) clinical isolates at Bugando medical centre in Mwanza.
Material and methods: The 70% methanol and distilled water A. indica leaf extracts were prepared. Ten UPEC clinical isolates were cultured into Muller Hinton agar with A. indica impregnated disks and other conventional antimicrobial disks. Zone of inhibition and minimum bacterial concentration (MBC) were obtained by using disc diffusion method and broth microdilution methods, respectively.
Results: The A. indica leaf 70% methanol extract showed antibacterial activity against 30% of ESBL producing UPEC at a concentration of 0.3g/ml. The MBC was at 0.6g/ml concentration. The diameter of inhibition of A. indica to 10 UPEC isolates ranged from 8 mm to 20 mm. The overall percentage of resistance of 10 UPEC isolates for other antibiotics were: meropenem (0%), gentamicin (70%), ciprofloxacin (90%), ceftazidime (100%) and trimethoprim sulfamethoxazole (100%).
Conclusion and recommendations: The A. indica leaf extract exhibited the anti-bacterial activity against 30% of ESBL producing E. coli at a concentration of 0.3g/ml. This antimicrobial effect was shown only 70% methanol extract and not with aqueous extract. Future to assess active ingredients and toxicological profile of A. indica extracts are recommended.
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