In Vitro Antifungal Activity of Allium Sativum (Garlic) against Candida Albicans Clinical Isolates in Mwanza Tanzania
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TextPublisher number: Wurzburg Road 35, BMC Premises, Post Code: 33102: P. O. Box 1464, Mwanza – Tanzania: Phone: +255 28 298 3384: Fax: +255 28 298 3386: Email: vc@bugando.ac.tzwww.bugando.ac.tzLanguage: English Language: Kiswahili Publication details: Mwanza, Tanzania : Catholic University of Health and Allied Sciences [CUHAS - Bugando ] : ©2018 Description: viii; 17 Pages; Includes ReferencesSubject(s): Summary: Abstract:
Background: Allium sativum contain the active chemical compounds such as Allicin and Ajoen. Antimicrobial anti-cancer and anti-thrombotic effect of Allium sativum have been documented previously C. Albicans is the leading opportunistic fungi pathogen with limited drug of choice. In Tanzania treatment of candidiasis main rely on azoles. The emergence of azole resistant among C. albicans call for the need of discovering new antifungal agents. The current study was conducted to evaluate the effect of Allium sativum on Candida albicans.
Method: It was laboratory experimental analysis which conducted from May to August 2018 which included 20 archived of Candida albicans. By agar dilution method Muller Hinton supplement with garlic extract of different concentrations were prepared following standard microbiological techniques. The plates containing Muller Hinton agar + Garlic were inoculated with C. Albicans isolates and incubated under appropriate conditions. The plate with growth was considered as resistant whereas minimum concentration that inhibits growth was recorded as MIC.
Results: In the current study C. albicans grew at the concentration of 2.5mg/ml up to 20mg/ml. At the concentration of 25mg/ml, 30mg/ml and 40mg/ml were 55% (11:20), 65% (13:20), 100% (20:20) and 100% (20:20) inhibit the growth respectively. The minimum inhibitory concentration was 35mg/ml.
Conclusion: Allium sativum extract inhibited fungal growth at a concentration of 35mg/ml. So the result of this study provides scientific justification for the use of garlic extract in health products and herbal medicine in treating fungal ailment especially due to Candida albicans
| Item type | Current library | Collection | Status | Barcode | |
|---|---|---|---|---|---|
| UNDERGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | 2 | UD0043 |
Abstract:
Background: Allium sativum contain the active chemical compounds such as Allicin and Ajoen. Antimicrobial anti-cancer and anti-thrombotic effect of Allium sativum have been documented previously C. Albicans is the leading opportunistic fungi pathogen with limited drug of choice. In Tanzania treatment of candidiasis main rely on azoles. The emergence of azole resistant among C. albicans call for the need of discovering new antifungal agents. The current study was conducted to evaluate the effect of Allium sativum on Candida albicans.
Method: It was laboratory experimental analysis which conducted from May to August 2018 which included 20 archived of Candida albicans. By agar dilution method Muller Hinton supplement with garlic extract of different concentrations were prepared following standard microbiological techniques. The plates containing Muller Hinton agar + Garlic were inoculated with C. Albicans isolates and incubated under appropriate conditions. The plate with growth was considered as resistant whereas minimum concentration that inhibits growth was recorded as MIC.
Results: In the current study C. albicans grew at the concentration of 2.5mg/ml up to 20mg/ml. At the concentration of 25mg/ml, 30mg/ml and 40mg/ml were 55% (11:20), 65% (13:20), 100% (20:20) and 100% (20:20) inhibit the growth respectively. The minimum inhibitory concentration was 35mg/ml.
Conclusion: Allium sativum extract inhibited fungal growth at a concentration of 35mg/ml. So the result of this study provides scientific justification for the use of garlic extract in health products and herbal medicine in treating fungal ailment especially due to Candida albicans
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