Abstract:

Background: Vulvovaginal candidiasis is a common disease among women and infection occur more frequently among pregnant women. Recurrent infection of among pregnant women recently has been increased; this might be due to reported increase of resistant Candida spp. This study aims at determining virulence and genotype distribution of candida colonizing vaginal of pregnant women with and without PROM.

Methodology: Retrievable isolates of Candida spp. Which were originally from pregnant women with and without PROM. Germ tube and CHROagar culture were used for speciation of Candida. Virulence factors including hydrolytic enzymes such as proteinase, phospholipase, coagulase and hemolytic activities was performed DNA from pure colonies was extracted and then 25 rDNA assay using arbitrary chosen primers will be performed.

Results: Forty two (42) isolates from pregnant women with and without PROM were included 25 were PROM+ and 17 were PROM-, all 42(100%) had hemolysis activities, twenty eight (66.7%) produced phospholipase, twenty one (50%) produced proteinase and fourteen (33.3%) indicated coagulase activities. Of all 42 isolates majority were genotype A (66.7%), and the remaining were genotype B and C indicating 19% and 14.3% respectively.

Conclusion: There was no statistical relevant deference of virulence activities and genotypes between pregnant women with PROM and without PROM. So we can say there is high virulence activities produced by C. albican from pregnant women with and without PROM, and they mostly express genotype A.