Draft genome sequence of a multidrug-resistant caprine isolate of Staphylococcus cohnii subsp. urealyticus from Tanzania encoding ermB, tet (K), dfrG, fusF and fosD
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TextPublisher number: Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz Language: English Series: ; Journal of global antimicrobial resistance Volume 18Publication details: Mwanza: Elsevier & Tanzania Catholic University of Health and Allied Sciences [CUHAS – Bugando] 2019/9/1Description: Pages 163-165Online resources: Summary: Abstract
Objectives: Coagulase-negative staphylococci such as Staphylococcus cohnii are opportunistic pathogens of humans and animals. A multidrug-resistant isolate of S. cohnii subsp. urealyticus (073AN) was isolated from the nasal cavity of a healthy goat in Tanzania. This study produced and analysed a draft genome sequence of strain 073AN to investigate the genetic basis for antimicrobial resistance in this isolate.
Methods: Strain 073AN was sequenced using HiSeq 2000 technology, sequencing reads were assembled using Velvet, and the genome was annotated using Prokka.
Results: The draft genome of strain 073AN is 2 677 652 bp in size with a GC content of 32.5%. The isolate was resistant to several classes of antimicrobials, which correlated with the presence of known antimicrobial resistance genes. Of particular note, the draft genome sequence of strain 073AN represents the first report of fosD in S. cohnii and the first descriptions of fosD and fusF in Africa.
Conclusion: The draft genome sequence of S. cohnii subsp. urealyticus 073AN released here provides an insight into the antimicrobial resistance determinants found in this species and in Tanzania and offers a valuable resource for further studies on staphylococcal genomics and antimicrobial resistance.
| Item type | Current library | Collection | Status | Barcode | |
|---|---|---|---|---|---|
| RESEARCH ARTICLES | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | -1 | RA0518 |
Abstract
Objectives: Coagulase-negative staphylococci such as Staphylococcus cohnii are opportunistic pathogens of humans and animals. A multidrug-resistant isolate of S. cohnii subsp. urealyticus (073AN) was isolated from the nasal cavity of a healthy goat in Tanzania. This study produced and analysed a draft genome sequence of strain 073AN to investigate the genetic basis for antimicrobial resistance in this isolate.
Methods: Strain 073AN was sequenced using HiSeq 2000 technology, sequencing reads were assembled using Velvet, and the genome was annotated using Prokka.
Results: The draft genome of strain 073AN is 2 677 652 bp in size with a GC content of 32.5%. The isolate was resistant to several classes of antimicrobials, which correlated with the presence of known antimicrobial resistance genes. Of particular note, the draft genome sequence of strain 073AN represents the first report of fosD in S. cohnii and the first descriptions of fosD and fusF in Africa.
Conclusion: The draft genome sequence of S. cohnii subsp. urealyticus 073AN released here provides an insight into the antimicrobial resistance determinants found in this species and in Tanzania and offers a valuable resource for further studies on staphylococcal genomics and antimicrobial resistance.
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