Sputum Induction For Microbiological Confirmation of Pulmonary Tuberculosis Among HIV-Infected Adults With Non -Productive Cough in Mwanza
Material type:
TextPublication details: Mwanza, Tanzania Catholic University of Health and Allied Sciences CUHAS - Bugando ©2015Description: xiii; 58 Pages; includes References and AppendicesSubject(s): Summary: Abstract:
Background: Patients suspected to have pulmonary tuberculosis (PTB) with a dry cough are difficult to diagnose. Sputum induction is one method to improve collection of sputum. Little is known about the feasibility or utility of sputum induction to increase the diagnostic yield of PTB among HIV-infected patients in Tanzania.
Objective: The objectives of this study was to investigate the yield of sputum induction and utility of gene Xpert among HIV-infected adults with non-productive cough in Mwanza, Tanzania.
Methodology: In this cross-sectional study, HIV-infected adults with non-productive cough suspected to have PTB were serially enrolled from Bugando Medical Centre, Sekou Toure, and Nyamagana hospital Sputum induction was performed, and a single induced sputum sample obtained. Sputum was analysed for acid-fast bacilli (AFB) by fluorescent microscope, geneXpert and Lowenstein-Jensen culture (LJ) culture.
Results: From January, 2015 until April, 2015 151 participants were enrolled. AFB were identified in 11/146 (7.5%) by fluorescent microscopy, 13/146 (8.9%) by geneXpert and 16/146 (11%) by LJ culture. GeneXpert had a sensitivity of 81.2% and specificity of 100% when compared to a gold standard of LJ culture. One patient was found to have rifampicin resistance by geneXpert MTB/RIF.
Conclusion: Sputum induction is safe and feasible and successfully increased the number of microbiologically confirmed cases of PTB. Sputum induction should be introduced as a routine method for to improve TB case finding among populations with dry cough such as HIV-infected individuals. It can increase number of TB cases detected in a timely manner, thus reducing morbidity and mortality.
| Item type | Current library | Collection | Status | Barcode | |
|---|---|---|---|---|---|
| POSTGRADUATE DISSERTATIONS | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | NFIC | 1 | PD0231 |
Abstract:
Background: Patients suspected to have pulmonary tuberculosis (PTB) with a dry cough are difficult to diagnose. Sputum induction is one method to improve collection of sputum. Little is known about the feasibility or utility of sputum induction to increase the diagnostic yield of PTB among HIV-infected patients in Tanzania.
Objective: The objectives of this study was to investigate the yield of sputum induction and utility of gene Xpert among HIV-infected adults with non-productive cough in Mwanza, Tanzania.
Methodology: In this cross-sectional study, HIV-infected adults with non-productive cough suspected to have PTB were serially enrolled from Bugando Medical Centre, Sekou Toure, and Nyamagana hospital Sputum induction was performed, and a single induced sputum sample obtained. Sputum was analysed for acid-fast bacilli (AFB) by fluorescent microscope, geneXpert and Lowenstein-Jensen culture (LJ) culture.
Results: From January, 2015 until April, 2015 151 participants were enrolled. AFB were identified in 11/146 (7.5%) by fluorescent microscopy, 13/146 (8.9%) by geneXpert and 16/146 (11%) by LJ culture. GeneXpert had a sensitivity of 81.2% and specificity of 100% when compared to a gold standard of LJ culture. One patient was found to have rifampicin resistance by geneXpert MTB/RIF.
Conclusion: Sputum induction is safe and feasible and successfully increased the number of microbiologically confirmed cases of PTB. Sputum induction should be introduced as a routine method for to improve TB case finding among populations with dry cough such as HIV-infected individuals. It can increase number of TB cases detected in a timely manner, thus reducing morbidity and mortality.
There are no comments on this title.
Click on an image to view it in the image viewer