Comparison of Modified Ultrafast Papanicolaou Staining Technique with Conventional Papanicolaou Staining Technique in Assessment of Cytological Samples at Bugando Medical Center in Mwanza, Tanzania. (Record no. 28736)

MARC details
000 -LEADER
fixed length control field 03455nam a22003137a 4500
001 - CONTROL NUMBER
control field 20240918111155.0
003 - CONTROL NUMBER IDENTIFIER
control field 20240918111155.0
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20240918111833.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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028 ## - PUBLISHER OR DISTRIBUTOR NUMBER
Source Wurzburg Road 35, Premises, Post Code: 33102 |
Source P. O. Box 1464 Mwanza, Tanzania |
Source Phone: (255) 28-298-3384 |
Source Fax: (255) 28-298-3386 |
Source Email: vc@bugando.ac.tz |
Source Website: www.bugando.ac.tz
040 ## - CATALOGING SOURCE
Transcribing agency ddc
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title English
Language code of text/sound track or separate title Kiswahili
100 ## - MAIN ENTRY--PERSONAL NAME
Relator term CUHAS/BM/1001226/T/21
Fuller form of name Gema Robert Massawe
245 ## - TITLE STATEMENT
Title Comparison of Modified Ultrafast Papanicolaou Staining Technique with Conventional Papanicolaou Staining Technique in Assessment of Cytological Samples at Bugando Medical Center in Mwanza, Tanzania.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Mwanza, Tanzania |
Name of publisher, distributor, etc. Catholic University of Health and Allied Sciences [CUHAS-Bugando] |
Date of publication, distribution, etc. 2024.
300 ## - PHYSICAL DESCRIPTION
Extent 40 Pages
Extent Includes References
520 ## - SUMMARY, ETC.
Summary, etc. Abstract:<br/><br/>Background: Cytological analysis plays a significant role in the early detection and diagnosis of several diseases, including cancer. The Papanicolaou (PAP) stain was developed by Dr. George N Papanicolaou, has long been considered the gold standard for screening and diagnosing cervical cancer and other cytological abnormalities. This method has some limitations including labor-intensive and time-consuming. To overcome these challenges modified ultrafast Pap staining methods have been developed.<br/><br/>Objective: To compare the staining quality of the Modified Ultrafast Papanicolaou with Conventional Papanicolaou Staining techniques in cytological samples at BMC.<br/><br/>Methods: This was a cross-sectional laboratory-based study conducted from July to August 2024 at BMC at Histopathology & morbid anatomy laboratory, convenience sampling technique was used to obtain cytological samples. Two smears were prepared for conventional PAP stain and MUFP stain technique, microscopic examination was done to compare the staining quality of the two techniques. Data was inserted into excel for cleaning and analyzed using STATA version 15, where Percentage or fraction were be used to summarize categorical variables while mean (STD)/median (IQR) was used for continuous variables. Ch2/Fisher’s exact was used to show association between parameters in conventional PAP stain and MUFP stain. P-value of <0.05 was considered as statistically significant.<br/><br/>Result: Out of 82 cytological samples, clean background stain was seen in 100% of smears stained by MUFP and hemorrhagic background was seen in 24.4% of PAP smears, majority of PAP smears 91.5% had crisp nucleus and 12.2% of MUFP has dull nucleus, 74.4% of MUFP smears showed well preserved cellular morphology, 53.7% of PAP smears had moderate preservation and 8.5% of MUFP smears showed no preservation of cellular morphology.<br/><br/>Conclusion: MUFP Stain shows a clear background & crisp nuclear features when compared to conventional PAP stain. Also, the preservation of cellular morphology and overall staining in MUFP is almost equal to that of conventional PAP stain <br/>
600 ## - SUBJECT ADDED ENTRY--PERSONAL NAME
General subdivision Pathology
700 ## - ADDED ENTRY--PERSONAL NAME
Fuller form of name Oscar Ottoman Muhini
856 ## - ELECTRONIC LOCATION AND ACCESS
Public note Research Report Submitted in Partial Fulfillment for The Award of Bachelor of Medical Laboratory Sciences Degree of Catholic University of Health and Allied Sciences.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme ddc
Koha item type UNDERGRADUATE DISSERTATIONS
Suppress in OPAC
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Date acquired Total checkouts Barcode Date last seen Price effective from Koha item type
          MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO 09/18/2024   20240918111155.0 09/18/2024 09/18/2024 UNDERGRADUATE DISSERTATIONS
Catholic University of  Health and Allied Sciences - CUHAS
Directorate of ICT @ 2024