Comparison Of Morphological Characteristics, Its Efficacy Of 10% Formic Acid and Cocktail Mixture of The Nitric Acid and EDTA in Decalcifying Bone Tissues at Bugando Medical Centre. (Record no. 28726)
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| fixed length control field | 03903nam a22003137a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 20240917115317.0 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | 20240917115317.0 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240917120355.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 240917b |||||||| |||| 00| 0 eng d |
| 028 ## - PUBLISHER OR DISTRIBUTOR NUMBER | |
| Source | Wurzburg Road 35, Premises, Post Code: 33102 | |
| Source | P. O. Box 1464 Mwanza, Tanzania | |
| Source | Phone: (255) 28-298-3384 | |
| Source | Fax: (255) 28-298-3386 | |
| Source | Email: vc@bugando.ac.tz | |
| Source | Website: www.bugando.ac.tz |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | ddc |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | English |
| Language code of text/sound track or separate title | Kiswahili |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Relator term | CUHAS/BM/1001217/T/21 |
| Fuller form of name | Joniah K. Paul |
| 245 ## - TITLE STATEMENT | |
| Title | Comparison Of Morphological Characteristics, Its Efficacy Of 10% Formic Acid and Cocktail Mixture of The Nitric Acid and EDTA in Decalcifying Bone Tissues at Bugando Medical Centre. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mwanza, Tanzania | |
| Name of publisher, distributor, etc. | Catholic University of Health and Allied Sciences [CUHAS-Bugando] | |
| Date of publication, distribution, etc. | 2024. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 43 Pages |
| Extent | Includes References |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Abstract:<br/><br/>Background: Bones are hard tissues forming the body skeleton, composed of 60% inorganic component (hydroxyapatite), 10% water and 30% organic component. In routine histopathology, the study of fibrillar, cellular and sub cellular structures of mineralized tissues like bones is only possible after removal of the calcium apatite of these hard tissues by the process of decalcification using different decalcifying agents such as acids or chelating agents. In our setting there is no study about comparing the efficacy of 10% formic acid and cocktail mixture of nitric acid and EDTA. The aim of the study was changing 10% formic acid the routine decalcifying agent to cocktail mixture.<br/><br/>Objectives: The study evaluated the efficacy of two different decalcifying agents by identifying fastest decalcifying agents, effects of agents on integrity of the bone tissues, and staining characteristics of the bones decalcified in the two different decalcifying agents.<br/><br/>Material and methodology: Prospective descriptive study was conducted on all bone tissues in Histopathology and Morbid anatomy Laboratory at BMC from June to July 2024. Total of 56 bone cases were studied which are skulls, ribs, clavicles, scapulars and teeth. For each case, that was obtained at histopathology laboratory was well fixed, then decalcified (using two decalcifying solutions were prepared), processed, embedded, sectioned, stained and observed microscopically according to BMC-Histopathology and morbid anatomy laboratory standard guidelines. The scores were given on five parameters: speed of decalcification, background, overall staining pattern, cell morphology and nuclear staining. <br/><br/>Results: A total number of 56 bone tissue samples were enrolled in this study, cocktail of Nitric acid and EDTA was the fastest agent which took 9.8 ± 6 days while 10% formic acid was slowest agent 15.2 ±5 days for complete decalcification, 10% formic acid shown best results compared to cocktail mixture on other parameters which are overall staining, background, cell morphology and nuclear character.<br/><br/>Conclusion: Histological sections impressions depend on a variety of factors which includes fixation, tissue processing, sectioning and staining timings. The final results led to the proposition that 10% formic acid was indeed the best decalcifying agent and should be used if time factor is not essential. In case of time become relevant for example if surgeon depend on pathologist output for patient management, cocktail of 10% nitric acid and 1% EDTA can be used due to its fastest action and good staining features.<br/> |
| 600 ## - SUBJECT ADDED ENTRY--PERSONAL NAME | |
| General subdivision | Pathology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Fuller form of name | Oscar Ottoman Muhini |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Public note | Research Report Submitted in Partial Fulfillment for The Award of Bachelor of Medical Laboratory Sciences Degree of Catholic University of Health and Allied Sciences. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | ddc |
| Koha item type | UNDERGRADUATE DISSERTATIONS |
| Suppress in OPAC | |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | 09/17/2024 | 20240917115317.0 | 09/17/2024 | 09/17/2024 | UNDERGRADUATE DISSERTATIONS |