Unraveling virulence determinants in extended-spectrum beta-lactamase-producing Escherichia coli from East Africa using whole-genome sequencing (Record no. 28094)
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| fixed length control field | 03434nam a22004097a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 20240612131137.0 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | 20240612131137.0 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240612133611.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
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| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1471-2334 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | ddc |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | English |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Fuller form of name | Ivan Sserwadda |
| 245 ## - TITLE STATEMENT | |
| Title | Unraveling virulence determinants in extended-spectrum beta-lactamase-producing Escherichia coli from East Africa using whole-genome sequencing |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mwanza, Tanzania : |
| Name of publisher, distributor, etc. | Catholic University of Health and Allied Sciences [CUHAS-Bugando] : |
| Date of publication, distribution, etc. | 2023 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | Pages 01-10 |
| Extent | Includes References |
| 490 ## - SERIES STATEMENT | |
| Volume/sequential designation | BMC Infectious Diseases volume 23, Article number: 587 (2023) |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Abstract : <br/><br/>Escherichia coli significantly causes nosocomial infections and rampant spread of antimicrobial resistance (AMR). There is limited data on genomic characterization of extended-spectrum β-lactamase (ESBL)-producing E. coli from African clinical settings. This hospital-based longitudinal study unraveled the genetic resistance elements in ESBL E. coli isolates from Uganda and Tanzania using whole-genome sequencing (WGS). A total of 142 ESBL multi-drug resistant E. coli bacterial isolates from both Tanzania and Uganda were sequenced and out of these, 36/57 (63.1%) and 67/85 (78.8%) originated from Uganda and Tanzania respectively. Mutations in RarD, yaaA and ybgl conferring resistances to chloramphenicol, peroxidase and quinolones were observed from Ugandan and Tanzanian isolates. We reported very high frequencies for blaCTX−M−15 with 11/18(61.1%), and blaCTX−M−27 with 12/23 (52.1%), blaTEM−1B with 13/23 (56.5%) of isolates originating from Uganda and Tanzania respectively all conferring resistance to Beta-lactam-penicillin inhibitors. We observed chloramphenicol resistance-conferring gene mdfA in 21/23 (91.3%) of Tanzanian isolates. Extraintestinal E. coli sequence type (ST) 131 accounted for 5/59 (8.4%) of Tanzanian isolates while enterotoxigenic E. coli ST656 was reported in 9/34 (26.4%) of Ugandan isolates. Virulence factors originating from Shigella dysenteriae Sd197 (gspC, gspD, gspE, gspF, gspG, gspF, gspH, gspI), Yersinia pestis CO92 (irp1, ybtU, ybtX, iucA), Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (csgF and csgG), and Pseudomonas aeruginosa PAO1 (flhA, fliG, fliM) were identified in these isolates. Overall, this study highlights a concerning prevalence and diversity of AMR-conferring elements shaping the genomic structure of multi-drug resistant E. coli in clinical settings in East Africa. It underscores the urgent need to strengthen infection-prevention controls and advocate for the routine use of WGS in national AMR surveillance and monitoring programs.<br/>. |
| 600 ## - SUBJECT ADDED ENTRY--PERSONAL NAME | |
| General subdivision | Extended-spectrum β-lactamase |
| General subdivision | East Africa |
| General subdivision | Whole-genome sequencing |
| General subdivision | Antimicrobial resistance |
| General subdivision | Virulence factors |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Fuller form of name | Benson R. Kidenya |
| Fuller form of name | Stephen Kanyerezi |
| Fuller form of name | Inyasi Lawrence Akaro |
| Fuller form of name | Baraka Mkinze |
| Fuller form of name | Stephen E. Mshana |
| Fuller form of name | Suhaila O. Hashim |
| Fuller form of name | Everlyne Isoe |
| Fuller form of name | Jeremiah Seni |
| Fuller form of name | Moses L. Joloba |
| Fuller form of name | Gerald Mboowa |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1186/s12879-023-08579-0">https://doi.org/10.1186/s12879-023-08579-0</a> |
| Link text | https://doi.org/10.1186/s12879-023-08579-0 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | ddc |
| Koha item type | RESEARCH ARTICLES |
| Suppress in OPAC | |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | 06/12/2024 | 20240612131137.0 | 06/12/2024 | 06/12/2024 | RESEARCH ARTICLES |