Prevalence of Glucose 6 Phosphate Dehydrogenase Deficiency, Degree of Anaemia among Sickle Cell Disease Patients Attending Clinic at Bugando Medical Centre Mwanza, Tanzania. (Record no. 22968)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04244nam a22003137a 4500 |
| 001 - CONTROL NUMBER | |
| control field | CUHAS/BM/1001023/T/20 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | CUHAS/BM/1001023/T/20 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240305194007.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 231101b |||||||| |||| 00| 0 eng d |
| 028 ## - PUBLISHER OR DISTRIBUTOR NUMBER | |
| Source | Phone: +255 28 298 3384 |
| Source | Fax: +255 28 298 3386 |
| Source | Email: vc@bugando.ac.tz |
| Source | Website: www.bugando.ac.tz |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | CUHAS/BM/1001023/T/20 |
| 040 ## - CATALOGING SOURCE | |
| Language of cataloging | English |
| Transcribing agency | DDC |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | English |
| Language code of text/sound track or separate title | Kiswahili |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Esther Germiah Mwenda |
| Dates associated with a name | CUHAS/BM/1001023/T/20 |
| 245 ## - TITLE STATEMENT | |
| Title | Prevalence of Glucose 6 Phosphate Dehydrogenase Deficiency, Degree of Anaemia among Sickle Cell Disease Patients Attending Clinic at Bugando Medical Centre Mwanza, Tanzania. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mwanza, Tanzania: |
| Name of publisher, distributor, etc. | Catholic University of Health and Allied Sciences [CUHAS – Bugando] : |
| Date of publication, distribution, etc. | ©2023 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 45 Pages |
| Extent | Includes References and Appendicies |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Abstract:<br/><br/>Background: Glucose-6-phosphate dehydrogenase (G6PD) is the cytoplasmic enzyme that is essential for an erythrocyte’s capacity to withstand oxidative stress, the deficiency of this enzyme leads to a buildup of free radical and insoluble hemoglobin within the cell. Precipitated hemoglobin is disruptive to the structure and function of the RBCs membrane and leads to increase membrane permeability, osmotic fragility, and cell rigidity. The compromised integrity of the RBCs membrane results in both intravascular hemolysis and rapid removal of this cell within the splenic pump. Sickle cell disease (SCD) is the most common inherited disorder which occurs when both parents have either sickle cell disease (two sickle cell genes) or sickle cell trait (one sickle gene). Both G6PD deficiency and SCD are prevalent in malaria-endemic regions, controversy however persists as to whether G6PD is commoner in SCD subjects since the occurrence of enzyme deficient state with a chronic hemolytic disorder like SCD could potential predisposes to fetal hemolytic episodes. There is however a dearth of pediatric studies on this subject. <br/><br/>Objectives: To determine the prevalence of Glucose-6-phosphate dehydrogenase (G6PD) deficiency, degree of anemia among sickle cell disease patients at BMC, Mwanza Tanzania. <br/><br/>Methodology: This cross-sectional study was conducted in the Department of Hematology and blood transfusion of Bugando Medical Centre, Mwanza Tanzania among the sickle cell disease confirmed patients. Screening test for G6PD activity and degree of anemia by hemoglobin concentration. Blood samples of not less than 6ml volume was collected in disodium ethylenediaminetetraacetic acid (EDTA) coated anticoagulant venipuncture vacuum tube Then G6PD qualitative screening test was performed where hemoglobin (red color) is oxidized to methemoglobin which is (brown in color) by sodium nitrate. Their redox dye, methylene blue activates the pentose phosphate pathway resulting in the enzymatic conversion of methemoglobin back to hemoglobin in blood with normal G6PD activity, in G6PD deficiency cells there is no enzymatic conversion to hemoglobin due to this blood sample retaining brown color.<br/><br/>Results: This study revealed that 21.25% (n=51) of patients with sickle cell disease were G6PD deficiency positive. The results indicate notable presence of this deficiency within the study population.This study has also found that patients with SCD with moderate to severe anemia were more likely to have G6PD deficiency, this is due to co-existence of two hemolytic disease. We also found that the degree of hemolysis, lower MCV, MCHC and high reticulocytes were more likely to be seen in patients with the two pathologies. <br/><br/>Conclusion: This study places the prevalence of G6PD-deficiency among sickle cell disease patient at 21.5% which indicates a significant of G6PD-deficieny among SCD patient. There was also significant difference between the RBC indices of G6PD-normal and G6PD-deficincy participant. This implies that G6PD-deficeincy may increase the severity of anemia in SCD patient.<br/> |
| 600 ## - SUBJECT ADDED ENTRY--PERSONAL NAME | |
| General subdivision | Pathology |
| General subdivision | Hematology |
| General subdivision | Medical Laboratory Sciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | ddc |
| Koha item type | UNDERGRADUATE DISSERTATIONS |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Date acquired | Total checkouts | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | 11/01/2023 | CUHAS/BM/1001023/T/2 | 11/01/2023 | 11/01/2023 | UNDERGRADUATE DISSERTATIONS |