Comparison study for staining quality and cellularity of 10% neutral buffered formalin over bouins solution in fixing fine needle aspiration samples for cell block techniques at Bugando Medical Centre in Mwanza, Tanzania (Record no. 22699)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03676nam a22002897a 4500 |
| 001 - CONTROL NUMBER | |
| control field | CUHAS/BM/1000639/T/19. |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | CUHAS/BM/1000639/T/19. |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240305193958.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 221213b |||||||| |||| 00| 0 eng d |
| 028 ## - PUBLISHER OR DISTRIBUTOR NUMBER | |
| Source | Phone: +255 28 298 3384 |
| Source | Fax: +255 28 298 3386 |
| Source | Email: vc@bugando.ac.tz |
| Source | Website: www.bugando.ac.tz |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | CUHAS/BM/1000639/T/19. |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | DDC |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | English |
| Language code of text/sound track or separate title | Kiswahili |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Anifa Awadhi Rwebangila |
| Dates associated with a name | CUHAS/BM/1000639/T/19. |
| 9 (RLIN) | 47332 |
| 245 ## - TITLE STATEMENT | |
| Title | Comparison study for staining quality and cellularity of 10% neutral buffered formalin over bouins solution in fixing fine needle aspiration samples for cell block techniques at Bugando Medical Centre in Mwanza, Tanzania |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mwanza, Tanzania: |
| Name of publisher, distributor, etc. | Catholic University of Health and Allied Sciences [CUHAS – Bugando] |
| Date of publication, distribution, etc. | 18.05.2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 48 Pages |
| Extent | Includes References and Appendices |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Abstract:<br/><br/>Background statement; Cell block (CB) technique refers to the processing of sediments, blood clots or grossly visible fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The CB can be used for ancillary studies like immunohistochemistry and molecular tests. Different fixatives can be used in CB preparation including Bouin’s solution and 10% NBF. Therefore, this study aim to give the similarities and differences in staining quality and cellularity of 10% NBF over Bouin’s solution in fixing Fine Needle Aspiration (FNA) samples for CB technique at BMC.<br/><br/>Objective of the study: To determine the comparison of staining quality and cellularity of 10% NBF over Bouin’s solution in fixing FNA samples for CB technique at BMC.<br/><br/>Methodology: Prospective descriptive study that was conducted from May to June 2022 at CPL in Histopathology unit BMC. Was involving cell block preparation through centrifugation process and microscopic observation for the blocks and convectional smear of minimum 67 samples from FNA biopsies received at BMC CPL. The results were interpreted according to the criteria which are intensity of stain and the histoscore percentages. The results were then be presented by using table forms.<br/><br/>Results: A 70 FNA samples were used in cell block preparation each fixed in both Bouin’s solution and 10% NBF separately. Samples fixed with Bouin’s solution having an average score of 8.4 (60%) while 10% NBF having an average score of 7 (50%) both being within an average range (6-14).Also, sensitivity and specificity of 10% NBF were 65.22% and 66.67% respectively. Prepared normal smears from 70 samples 60 (85.7%) samples were diagnostically suitable while 10 (14.3%) samples were diagnostically unsuitable giving the sensitivity and specificity of 72.4% and 83.3% respectively.<br/><br/>Conclusion: In cytological smears fixed with 10% NBF for cell block preparation about half of the cytological specimen were positive average score. In cytological smear fixed with Bouin’s solution for cell block preparation two third of the cytological specimen were positive average score. Both 10% NBF and Bouin’s solution were within the average score range of histological score. The sensitivity and specificity of convectional normal smear for detection of cytological diagnosis was higher. Therefore, 10% NBF can be appropriate alternative to Bouin’s solution for fixation of FNA samples in cell block preparation at Central Pathology Laboratory, BMC <br/> |
| 600 ## - SUBJECT ADDED ENTRY--PERSONAL NAME | |
| General subdivision | Pathology |
| 9 (RLIN) | 15818 |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 30304 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | ddc |
| Koha item type | UNDERGRADUATE DISSERTATIONS |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | 12/13/2022 | CRECU/2325 | 12/13/2022 | CRECU/2325 | 12/13/2022 | UNDERGRADUATE DISSERTATIONS |