Liraglutide protects pancreatic β cells from endoplasmic reticulum stress by upregulating MANF to promote autophagy turnover (Record no. 19882)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03837nam a22003257a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240305193743.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 221202b |||||||| |||| 00| 0 eng d |
| 028 ## - PUBLISHER OR DISTRIBUTOR NUMBER | |
| Source | Phone: +255 28 298 3384 |
| Source | Fax: +255 28 298 3386 |
| Source | Email: vc@bugando.ac.tz |
| Source | Website: www.bugando.ac.tz |
| 040 ## - CATALOGING SOURCE | |
| Language of cataloging | English |
| Transcribing agency | DLC |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | English |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Jili Fu |
| 9 (RLIN) | 46506 |
| 210 ## - ABBREVIATED TITLE | |
| Abbreviated title | Abbreviations AKTserine/threonine kinase ATF4activating transcription factor 4 ATF6activating transcription factor 6 BSAbull serum albumin C-cas-3cleaved-caspase-3 CCK-8cell counting kit-8 CHOPC/EBP homologous protein CQchloroquine DMSOdimethyl sulfoxide ECLenhanced chemiluminescence ERendoplasmic reticulum FFAfree fatty acid GLUglucose HLDhyperlipidemia IRE1αinositol-requiring enzyme 1α LC3Bmicrotubule-associated protein 1 light chain 3B LRGliraglutide MANFmesencephalic astrocyte derived neurotrophic factor mTORmammalian target of rapamycin PApalmitate p-AKTphosphorylation serine/threonine kinase PBSphosphate buffered saline p-mTORmammalian target phosphorylated of rapamycin PMSFphenylrnethanesulfony1 fluoride p62/SQSTM1sequestosome 1 PVDFpolyvinylidene difluoride rpmrevolutions per minute T2Dtype 2 diabetes TBStri buffered saline TBSTtris-buffered saline with tween TEMtransmission electron microscope TGthapsigargin UPRunfolded protein response XBP-1sX-box binding protein 1 spliced |
| 222 ## - KEY TITLE | |
| Key title | Liraglutide MANF Autophagy Endoplasmic reticulum stress β cells |
| 245 ## - TITLE STATEMENT | |
| Title | Liraglutide protects pancreatic β cells from endoplasmic reticulum stress by upregulating MANF to promote autophagy turnover |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mwanza, Tanzania: |
| Name of publisher, distributor, etc. | Pergamon & |
| -- | Catholic University of Health and Allied Sciences [CUHAS – Bugando] |
| Date of publication, distribution, etc. | 2020/7/1 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | Pages 117648 |
| 490 ## - SERIES STATEMENT | |
| Volume/sequential designation | Life sciences Volume 252 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Abstract:<br/><br/>Aims: This study was conducted to determine the relationship between mesencephalic astrocyte-derived neurotrophic factor (MANF), autophagy and endoplasmic reticulum (ER) stress, and whether liraglutide (LRG) can protect β cells, promote autophagy and alleviate ER stress by regulating MANF expression.<br/><br/>Main methods: Human serum samples were collected from healthy controls (NC), simple hyperlipidemia (HLD), and newly diagnosed type 2 diabetes (T2D). The MANF levels were detected using ELISA. In vitro, after the mouse islet MIN6 cells were treated with glucose (GLU), palmitate (PA), thapsigargin (TG), LRG, and chloroquine (CQ), cell proliferation was detected using cell counting kit-8 (CCK-8), apoptosis-related protein cleaved caspase 3 (C-cas-3), ER stress, and autophagy-related proteins were detected by Western blotting, MANF, insulin, and C-cas-3 proteins were detected via immunofluorescence. Subcellular structures and autophagosomes were examined using electron microscopy.<br/><br/>Key findings: Compared with the NC group, the MANF levels in the HLD and T2D groups increased significantly. After ER stress induced by GLU, PA, and TG, cell viability decreased, while MANF, c-cas3, ERS, and autophagy-related proteins increased, which was related to the concentration of GLU, PA, and TG. Compared with the BSA group, the number of mitochondria and autophagosomes in the PA group increased and the mitochondria were damaged. In the PA and TG plus CQ groups, the effect was further exaggerated. But after co-treatment with LRG, the effects of GLU, PA, and TG were attenuated.<br/><br/>Significance: LRG protects islet β cells from ER stress by upregulating MANF to promote autophagy turnover. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 46507 |
| 9 (RLIN) | 46508 |
| 9 (RLIN) | 46509 |
| 9 (RLIN) | 46510 |
| 9 (RLIN) | 46511 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1016/j.lfs.2020.117648">https://doi.org/10.1016/j.lfs.2020.117648</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | ddc |
| Koha item type | RESEARCH ARTICLES |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | 12/02/2022 | RA1090 | 12/02/2022 | RA1090 | 12/02/2022 | RESEARCH ARTICLES |