Conjugative IncFI plasmids carrying CTX-M-15 among Escherichia coliESBL producing isolates at a University hospital in Germany (Record no. 19529)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03016nam a22003257a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240305193731.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 221121b |||||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1471-2334 |
| 028 ## - PUBLISHER OR DISTRIBUTOR NUMBER | |
| Source | Phone: +255 28 298 3384 |
| Source | Fax: +255 28 298 3386 |
| Source | Email: vc@bugando.ac.tz |
| Source | Website: www.bugando.ac.tz |
| 040 ## - CATALOGING SOURCE | |
| Language of cataloging | English |
| Transcribing agency | DLC |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | English |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Stephen E Mshana |
| 9 (RLIN) | 15820 |
| 222 ## - KEY TITLE | |
| Key title | Cefepime Tigecycline Cefpodoxim ESBL Gene ESBL Type |
| 245 ## - TITLE STATEMENT | |
| Title | Conjugative IncFI plasmids carrying CTX-M-15 among Escherichia coliESBL producing isolates at a University hospital in Germany |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mwanza, Tanzania: |
| Name of publisher, distributor, etc. | BioMed Central & |
| -- | Catholic University of Health and Allied Sciences [CUHAS – Bugando] |
| Date of publication, distribution, etc. | 17 June 2009 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | Pages 1-8 |
| 490 ## - SERIES STATEMENT | |
| Volume/sequential designation | BMC infectious diseases Volume 9 Issue 1 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Abstract<br/><br/>Background: Multi-drug-resistant, extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, constitute an emerging public-health concern. Little data on the molecular epidemiology of ESBL producing Escherichia coli is available in Germany. Here we describe the prevalence and molecular epidemiology of ESBL producing-Escherichia coli isolates at a German University hospital.<br/><br/>Methods: We analysed 63 non-duplicate clinical ESBL isolates obtained over an 8-month period using PCR and sequence-based ESBL allele typing, plasmid replicon typing, phylogenetic group typing. Pulsed-field gel electrophoresis (PFGE) based genotyping and plasmid profiling was performed, as well as confirmatory DNA-based hybridization assays.<br/><br/>Results: Examination of the 63 Escherichia coli isolates revealed an almost equal distribution among the E. coli phylogenetic groups A, B1, B2 and D. High prevalence (36/63) of the CTX-M-15 gene was observed and an analysis of PFGE-based patterns revealed the presence of this CTX-M allele in multiple clones. Resistance to cefotaxime was a transferable trait and a commonly occurring 145.5 kb conjugative IncFI plasmid was detected in 65% of E. coli carrying the CTX-M-15 allele. The rate of transferable antibiotic resistances for GM, SXT, TET, GM-SXT-TET, SXT-TET and GM-TET was 33%, 61%, 61%, 27%, 44% and 11%, respectively. The remaining strains did not have a common IncFI plasmid but harboured transferable IncFI plasmids with sizes that ranged from 97 to 242.5 kb.<br/><br/>Conclusion: Our data demonstrate the presence of IncFI plasmids within the prevailing E. coli population in a hospital setting and suggest that the dissemination of CTX-M-15 allele is associated to lateral transfer of these well-adapted, conjugative IncFI plasmids among various E. coli genotypes. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15822 |
| 9 (RLIN) | 45578 |
| 9 (RLIN) | 23625 |
| 9 (RLIN) | 23626 |
| 9 (RLIN) | 23153 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1186/1471-2334-9-97">https://doi.org/10.1186/1471-2334-9-97</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | ddc |
| Koha item type | RESEARCH ARTICLES |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | 11/21/2022 | RA0737 | 11/21/2022 | 11/21/2022 | RESEARCH ARTICLES |