The use of 0.01 M phosphate buffered saline as detection buffer for Alere Determine® HIV rapid test in resource limited settings (Record no. 19497)

MARC details
000 -LEADER
fixed length control field 02740nam a22003377a 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20240305193730.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 221121b |||||||| |||| 00| 0 eng d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number eISSN: 1821-9241
International Standard Serial Number print ISSN: 1821-6404
028 ## - PUBLISHER OR DISTRIBUTOR NUMBER
Source Phone: +255 28 298 3384
Source Fax: +255 28 298 3386
Source Email: vc@bugando.ac.tz
Source Website: www.bugando.ac.tz
040 ## - CATALOGING SOURCE
Transcribing agency DLC
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title English
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Mariam M Mirambo
9 (RLIN) 22927
222 ## - KEY TITLE
Key title Phosphate buffered saline, Alere Determine, rapid test, HIV
245 ## - TITLE STATEMENT
Title The use of 0.01 M phosphate buffered saline as detection buffer for Alere Determine® HIV rapid test in resource limited settings
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Mwanza, Tanzania:
Name of publisher, distributor, etc. Tanzania Journal of Health Research &
-- Catholic University of Health and Allied Sciences [CUHAS – Bugando]
Date of publication, distribution, etc. 2017/4/8
490 ## - SERIES STATEMENT
Volume/sequential designation Tanzania Journal of Health Research Volume 19 Issue 2
520 ## - SUMMARY, ETC.
Summary, etc. Abstract<br/><br/>Insufficient supply of manufacture’s buffers/diluents in relation to the number of strips per kit has been found to have negative impact on patients’ results. Some laboratories personnel tend to use diluents from other rapid tests manufacturers such as Bioline, Unigold as well as malaria rapid diagnostic test (MRDT). This study aimed at evaluating the use of 0.01M phosphate buffered saline (PBS) as detection buffer for Alere Determine® HIV rapid test. This study was carried out at Bugando School of Medicine in Mwanza, Tanzania. A total of 300 whole blood specimens; 150 HIV positive specimens from patients attending Care and Treatment Centreand 150 HIV negative specimens were retested for HIV status using Alere Determine® HIV rapid test employing normal Alere buffer and 0.01M PBS as buffer.Of the total specimens tested; 150 (100%) of HIV positive were positive by using both Alere buffer and 0.01M PBS while 150(100%) of HIV negative samples were negative by both Alere Determine® and 0.01M PBS. The agreement between 0.01M PBS and Alere Determine® buffer was 100%. The value of kappa indicates perfect agreement between 0.01M PBS and Alere Determine® buffer (100%). A 0.01M PBS is recommended as alternative detection buffer for Alere Determine® in cases of insufficient supply. Further investigation to evaluate the suitable buffer for other rapid tests for HIV and other diseases is recommended especially in resource limited settings.
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 19663
9 (RLIN) 45532
9 (RLIN) 45511
9 (RLIN) 22909
9 (RLIN) 22970
9 (RLIN) 15820
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://doi.org/10.4314/thrb.v19i2.10">https://doi.org/10.4314/thrb.v19i2.10</a>
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme ddc
Koha item type RESEARCH ARTICLES
Holdings
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