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Existence of Multiple ESBL Genes among Phenotypically Confirmed ESBL Producing Klebsiella pneumoniae and Escherichia coli Concurrently Isolated from Clinical, Colonization and Contamination Samples from Neonatal Units at Bugando Medical Center, Mwanza, Tanzania (Record no. 19005)

MARC details
000 -LEADER
fixed length control field	02799nam a22002657a 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field	210825b \|\|\|\|\|\|\|\| \|\|\|\| 00\| 0 eng d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number	EISSN 2079-6382
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name	Vitus Silago
9 (RLIN)	19665
222 ## - KEY TITLE
Key title	antimicrobial resistance; extended-spectrum beta-lactamase; Escherichia coli; Klebsiella pneumoniae
245 ## - TITLE STATEMENT
Title	Existence of Multiple ESBL Genes among Phenotypically Confirmed ESBL Producing Klebsiella pneumoniae and Escherichia coli Concurrently Isolated from Clinical, Colonization and Contamination Samples from Neonatal Units at Bugando Medical Center, Mwanza, Tanzania
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc.	Mwanza, Tanzania:
Name of publisher, distributor, etc.	Catholic University of Health and Allied Sciences CUHAS - Bugando &
--	MDPI
Date of publication, distribution, etc.	21 April 2021
300 ## - PHYSICAL DESCRIPTION
Extent	Pages 476
490 ## - SERIES STATEMENT
Materials specified	Antibiotics 2021, 10(5), 476
Series statement	Antibiotics 2021, 10(5), 476
Volume/sequential designation	Antibiotics Volume 10 Issue 5
520 ## - SUMMARY, ETC.
Summary, etc.	Abstract<br/><br/>The proportions and similarities of extended-spectrum β-lactamase (ESBL) producing K. pneumoniae (ESBL-KP) and E. coli (ESBL-EC) carrying multiple ESBL genes is poorly known at our setting. This study investigated the existence of multiple ESBL genes (blaCTX-M, blaTEM, and blaSHV) among ESBL-KP and ESBL-EC concurrently isolated from clinical, colonization, and contamination samples from neonatology units in Mwanza-Tanzania. Twenty and 55 presumptive ESBL-EC and ESBL-KP, respectively, from a previous study archived at −80 °C were successfully recovered for this study. Isolates were screened and confirmed for production of ESBLs by phenotypic methods followed by multiplex PCR assay to determine ESBL genes. All (100%) and 97.3% of presumptive ESBL isolates were phenotypically confirmed by Clinical and Laboratory Standards Institute (CLSI) and modified double-disc synergy methods, respectively. About 93.3% (70/75) of phenotypically confirmed ESBL isolates had at least one ESBL gene, whereby for 62.9% (44/70), all ESBL genes (blaCTX-M, blaTEM, and blaSHV) were detected. Eight pairs of ESBL bacteria show similar patterns of antibiotics susceptibility and ESBL genes. ESBL-KP and ESBL-EC, concurrently isolated from clinical, colonization and contamination samples, harbored multiple ESBL genes. Further, eight pairs of ESBL isolates had similar patterns of antibiotics susceptibility and ESBL genes, suggesting transmission of and/or sharing of mobile genetic elements (MGEs) among ESBL-KP and ESBL-EC.<br/><br/>
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN)	15820

9 (RLIN)	23302

9 (RLIN)	23301

9 (RLIN)	23299

9 (RLIN)	23298

9 (RLIN)	19633

9 (RLIN)	23622

9 (RLIN)	23297
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier	<a href="https://doi.org/10.3390/antibiotics10050476 ">https://doi.org/10.3390/antibiotics10050476 </a>
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme	ddc
Koha item type	RESEARCH ARTICLES

Holdings
Withdrawn status	Lost status	Source of classification or shelving scheme	Damaged status	Not for loan	Collection	Home library	Current library	Shelving location	Date acquired	Total checkouts	Barcode	Date last seen	Price effective from	Koha item type
						MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO	MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO		08/25/2021		RA0206	08/25/2021	08/25/2021	RESEARCH ARTICLES