Evaluation of detection methods for Campylobacter infections among under-fives in Mwanza City, Tanzania (Record no. 18991)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02512nam a22002177a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210825b |||||||| |||| 00| 0 eng d |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Martha Fidelis Mushi |
| 9 (RLIN) | 23600 |
| 245 ## - TITLE STATEMENT | |
| Title | Evaluation of detection methods for Campylobacter infections among under-fives in Mwanza City, Tanzania |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mwanza, Tanzania |
| Name of publisher, distributor, etc. | Catholic University of Health and Allied Sciences CUHAS - Bugando |
| Date of publication, distribution, etc. | 2014 |
| 490 ## - SERIES STATEMENT | |
| Materials specified | Pan Afr Med J. 2014; 19: 392. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Abstract<br/><br/>Introduction<br/><br/>Campylobacter species are recognized as a major cause of acute gastroenteritis in humans throughout the world. The diagnosis is mainly based on stool culture. This study was done to evaluate the effectiveness of staining methods (Gram stain using 0.3% carbol fuchsin as counter stain and 1% carbol fuchsin direct stain) versus culture as the gold standard.<br/><br/>Methods<br/><br/>A total of 300 children attending Bugando Medical Centre (BMC) and the Sekou Toure regional hospital with acute watery diarrhea were enrolled. Two sets of slides were prepared stained with 1% carbol fuchsin for 30 seconds first set, and the second set stained with Gram's stain using 0.3% carbol fuchsin as counter stain for five minutes. Concurrently, stool samples were inoculated on Preston Agar selective.<br/><br/>Results<br/><br/>Of 300 stool specimens, 14(4.7%) showed positive culture after 48 hours of incubation and 28 (9.3%) shows typical morphology of Campylobacter species by both Gram stain and direct stain. The sensitivity of the Gram stain using 0.3% carbol fuchsin as counter stain and 1% carbol fuchsin simple stain versus culture as gold standard was 64.3%, with a specificity of 93.4%. The positive predictive value and negative predictive value were 32.1% and 98.2% respectively.<br/><br/>Conclusion<br/><br/>The detection of Campylobacter by 1% carbol fuchsin is simple, inexpensive, and fast, with both a high sensitivity and specificity. Laboratories in settings with high prevalence of campylobacteriosis and/or limited resources can employ 1% carbol fuchsin direct stain in detecting campylobacter infections.<br/><br/>Keywords: Campylobacteriosis, acute watery diarrhea, gram stain, 1% carbol fuchsin, preston agar |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 23601 |
| 9 (RLIN) | 22640 |
| 9 (RLIN) | 23602 |
| 9 (RLIN) | 22727 |
| 9 (RLIN) | 19633 |
| 9 (RLIN) | 23603 |
| 9 (RLIN) | 23604 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | doi: 10.11604/pamj.2014.19.392.4242 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | ddc |
| Koha item type | RESEARCH ARTICLES |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | MWALIMU NYERERE LEARNING RESOURCES CENTRE-CUHAS BUGANDO | 08/25/2021 | RA0192 | 08/25/2021 | 08/25/2021 | RESEARCH ARTICLES |