Stepwise Culture of Human Adult Stem Cells.
Stepwise Culture of Human Adult Stem Cells. - Alsbjergvej 10, 9260 Gistrup, Denmark | River Publishers | 2024. - 177 Pages
This volume on stepwise techniques to culture adult human stem cells begins with the basic methods to culture adult stem cells followed by related methods to supplement stem cell culture. This and future volumes of techniques will allow laboratories across scientific entities – academic and commercial – to adapt the described methods for similar methods with cells from other species. The standardized techniques will allow across the board interpretation of other technologies such as Omics studies. These methods can be a starting platform for culturing stem cells even in laboratories that might not be familiar with the techniques.
Scientific and early clinical trials strongly favor the development of translational studies with adult stem cells. A major impediment to progress in the field of regenerative medicine is a lack of standardized methods to culture specific stem cells. Such documented methods would allow for scientific rigor and reproducibility to enhance efficient and safe translation of stem cells to patients. This book fills that gap.
978-87-7022-854-1 (hardback) 978-87-7004-097-6 (paperback) 978-10-4001-077-8 (online) 978-1-003-46687-1 (ebook master) = Stepwise Culture of Human Adult Stem Cells acute myeloid leukemia adipocytes antibodies BM bone marrow aspirate Cancer stem cells Cat cell lines cellular centrifuge clinical clone collagenase cryopreservation CSCs culture dishes dental pulp dental pulp stem Department of Medicine differentiation Division of Hematology/Oncology DMEM donor DPSCs expression FBS Fetal bovine serum fibroblasts Ficoll flow cytometry gate gene Graduate Studies hematopoietic cells hematopoietic stem cells HSCs hUCB incubate iPSCs isolation Jersey Medical School L-glutamine laboratory layer Lin LSC markers mesenchymal stem cells method ml mononuclear cells MPB MRD MSC media MSCs multipotent needle P-MSCs PBS pellet phenotype phosphate buffered saline placenta plate pluripotent stem cells population Pranela Rameshwar protocol pulp stem cells Ratajczak Reagents red blood cells regenerative medicine resuspend Rutgers New Jersey Rutgers School sample School of Graduate Sherman somatic cells SORE6-GFP sorting stem cells MSCs sterile stromal cells supernatant syringe ThermoFisher tissue culture trypsin tube UC umbilical cord umbilical cord blood volume VSELs Yamanaka factors µm
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