Level of skin colonization and clearance rate of Staphylococcus species before and after application of Povidone Iodine paint on patients undergoing major surgery at Bugando Medical Centre, Mwanza, Tanzania
Suzana R Massawe CUHAS/BP/3000547/T/18
Level of skin colonization and clearance rate of Staphylococcus species before and after application of Povidone Iodine paint on patients undergoing major surgery at Bugando Medical Centre, Mwanza, Tanzania - Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS – Bugando] ©13.09.2022 - Pages x; 36 Includes Refferences and Appendices
ABSTRACT:
Introduction: Antiseptics are substances that stop or slow down the growth of microorganism in or on living tissues. Applications of antiseptics before surgical procedures minimize the risk of developing Surgical Site Infections (SSIs). Povidone Iodine (PI) and PI paints are used in preoperative surgical skin preparation. The use of PI and Methylated Spirit (MS) has a synergistic effect thus bring a significant reduction of SSI, compared to PI alone.
Methodology: A cross sectional study involved patients undergoing major surgery in the main operating theatre at BMC which aimed at determining the level of skin colonization through culture results from skin swabs collected before antiseptic application and the clearance rate of Staphylococcus species on PI and PI paints determined through culture results from skin swabs collected after antiseptic application on patients undergoing major surgery at BMC.
Results: In culture results from skin colonization before antiseptic application three bacteria species were isolated with S.epidermidis most predominant 74/127 (58%) followed by S.saprophyticus 44/127 (35%) and S.aureus 9/127 (7%).The use of PI paints as antiseptics used in surgical skin preparation was effective in clearing normal skin flora by 81/81 (100%). Meanwhile the use of 10% PI alone had a clearance rate of 37/39 (95%) of normal skin flora and 2/39 (5%) showed positive bacterial growth (p=0.04) and the bacteria isolated was S. epidermidis.
Conclusion: The most commonly isolated skin flora was S.epidermidis 74/127 (58%), this is normal since it covers about 90% of the body’s normal flora. The use of 10% PI with 70% MS during surgical procedures has shown to be superior 100% than PI alone 95% thus, more studies must be done on the effectiveness of PI alone.
= OPERATIONAL DEFINITIONS Antiseptics Substances that inhibit growth of microbes OPERATIONAL DEFINITIONS Clearance rate Rate at which Staphylococcus species are removed from the skin after application of Povidone iodine or Povidone iodine paints OPERATIONAL DEFINITIONS Level of skin colonization Presence of Staphylococcus species on the skin at the site of incision after application of Povidone iodine or Povidone iodine paints OPERATIONAL DEFINITIONS Surgical Site Infection (SSI) Infection that occurs after surgery at the site of incision = ABBREVIATIONS BMC Bugando Medical Centre CUHAS Catholic University of Health and Allied Sciences MRSA Methicillin Resistant Staphylococcus aureus MSSA Methicillin Susceptible Staphylococcus aureus MS Methylated Spirit PI Povidone Iodine SSI Surgical Site Infection
Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz
-- Microbiology and Immunology--Surgery--Pharmacy
Level of skin colonization and clearance rate of Staphylococcus species before and after application of Povidone Iodine paint on patients undergoing major surgery at Bugando Medical Centre, Mwanza, Tanzania - Mwanza, Tanzania: Catholic University of Health and Allied Sciences [CUHAS – Bugando] ©13.09.2022 - Pages x; 36 Includes Refferences and Appendices
ABSTRACT:
Introduction: Antiseptics are substances that stop or slow down the growth of microorganism in or on living tissues. Applications of antiseptics before surgical procedures minimize the risk of developing Surgical Site Infections (SSIs). Povidone Iodine (PI) and PI paints are used in preoperative surgical skin preparation. The use of PI and Methylated Spirit (MS) has a synergistic effect thus bring a significant reduction of SSI, compared to PI alone.
Methodology: A cross sectional study involved patients undergoing major surgery in the main operating theatre at BMC which aimed at determining the level of skin colonization through culture results from skin swabs collected before antiseptic application and the clearance rate of Staphylococcus species on PI and PI paints determined through culture results from skin swabs collected after antiseptic application on patients undergoing major surgery at BMC.
Results: In culture results from skin colonization before antiseptic application three bacteria species were isolated with S.epidermidis most predominant 74/127 (58%) followed by S.saprophyticus 44/127 (35%) and S.aureus 9/127 (7%).The use of PI paints as antiseptics used in surgical skin preparation was effective in clearing normal skin flora by 81/81 (100%). Meanwhile the use of 10% PI alone had a clearance rate of 37/39 (95%) of normal skin flora and 2/39 (5%) showed positive bacterial growth (p=0.04) and the bacteria isolated was S. epidermidis.
Conclusion: The most commonly isolated skin flora was S.epidermidis 74/127 (58%), this is normal since it covers about 90% of the body’s normal flora. The use of 10% PI with 70% MS during surgical procedures has shown to be superior 100% than PI alone 95% thus, more studies must be done on the effectiveness of PI alone.
= OPERATIONAL DEFINITIONS Antiseptics Substances that inhibit growth of microbes OPERATIONAL DEFINITIONS Clearance rate Rate at which Staphylococcus species are removed from the skin after application of Povidone iodine or Povidone iodine paints OPERATIONAL DEFINITIONS Level of skin colonization Presence of Staphylococcus species on the skin at the site of incision after application of Povidone iodine or Povidone iodine paints OPERATIONAL DEFINITIONS Surgical Site Infection (SSI) Infection that occurs after surgery at the site of incision = ABBREVIATIONS BMC Bugando Medical Centre CUHAS Catholic University of Health and Allied Sciences MRSA Methicillin Resistant Staphylococcus aureus MSSA Methicillin Susceptible Staphylococcus aureus MS Methylated Spirit PI Povidone Iodine SSI Surgical Site Infection
Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz
-- Microbiology and Immunology--Surgery--Pharmacy