In Vitro Investigation of Natural Plant Bidens Pilosa Against Candida Species Clinical Isolates in Mwanza, Tanzania.
Mwanyika, Leticia CUHAS/BP/300084/T/12
In Vitro Investigation of Natural Plant Bidens Pilosa Against Candida Species Clinical Isolates in Mwanza, Tanzania. - Mwanza, Tanzanaia: Catholic University of Health and Allied Sciences [CUHAS - Bugando] : Phone: +255 28 298 3384 : Fax: +255 28 298 3386 : Email: vc@bugando.ac.tz : Website: www.bugando.ac.tz : ©16.08.2016 - xi; 19 Pages Includes References and Appendices
Abstract:
Background: An enormous variety of medicinal plants are used worldwide by about 80% of the world population, although in most cases no scientific studies have done to prove the efficacy of these medicinal plants of European, Mediterranean and Arabic origin, the variety of plants in use around the world may very well represent an enormous treasure for drug development. Due to limited information on the therapeutic utility of various plants in Tanzania, the main objective of this study was to investigate the antimicrobial activity of Bidens pilosa against Candida species.
Methodology: This experimental analytical study was conducted from April to June 2016. The fresh leaves was collected in Mwanza, and using standard operating procedures in the CUHAS Multipurpose laboratory. Using aseptic techniques, the plants were grinded in, then squeezed to obtain the juice and filtered by the use of Withman filter paper. Plain antimicrobial discs were soaked into extracts of the leaves, dried and then, used for antifungal activities’ screening for 22 Candida spp agar diffusion method.
Results: The juice from the B.pilosa fresh leaves was able to inhibit growth of Candida spp. With zone of inhibition between 12mm-36mm and the minimum inhibitory volume of fresh juice which was able to inhibit growth was ≥ 6.25/100ml. The sensitivity for C.albicans was 77.2%, 81.8%, 22.7% and 18.2% to B.pilosa, Nystatin 100 units, fluconazole and Amphotericin respectively.
Conclusion: This study has shown potential antifungal activity of B.pilosa plant leaves against Candida spp. So, the need for more analytical studies to ascertain the active ingredient conferring this antifungal is recommended.
--Pharmacy --Microbiology and Immunology
In Vitro Investigation of Natural Plant Bidens Pilosa Against Candida Species Clinical Isolates in Mwanza, Tanzania. - Mwanza, Tanzanaia: Catholic University of Health and Allied Sciences [CUHAS - Bugando] : Phone: +255 28 298 3384 : Fax: +255 28 298 3386 : Email: vc@bugando.ac.tz : Website: www.bugando.ac.tz : ©16.08.2016 - xi; 19 Pages Includes References and Appendices
Abstract:
Background: An enormous variety of medicinal plants are used worldwide by about 80% of the world population, although in most cases no scientific studies have done to prove the efficacy of these medicinal plants of European, Mediterranean and Arabic origin, the variety of plants in use around the world may very well represent an enormous treasure for drug development. Due to limited information on the therapeutic utility of various plants in Tanzania, the main objective of this study was to investigate the antimicrobial activity of Bidens pilosa against Candida species.
Methodology: This experimental analytical study was conducted from April to June 2016. The fresh leaves was collected in Mwanza, and using standard operating procedures in the CUHAS Multipurpose laboratory. Using aseptic techniques, the plants were grinded in, then squeezed to obtain the juice and filtered by the use of Withman filter paper. Plain antimicrobial discs were soaked into extracts of the leaves, dried and then, used for antifungal activities’ screening for 22 Candida spp agar diffusion method.
Results: The juice from the B.pilosa fresh leaves was able to inhibit growth of Candida spp. With zone of inhibition between 12mm-36mm and the minimum inhibitory volume of fresh juice which was able to inhibit growth was ≥ 6.25/100ml. The sensitivity for C.albicans was 77.2%, 81.8%, 22.7% and 18.2% to B.pilosa, Nystatin 100 units, fluconazole and Amphotericin respectively.
Conclusion: This study has shown potential antifungal activity of B.pilosa plant leaves against Candida spp. So, the need for more analytical studies to ascertain the active ingredient conferring this antifungal is recommended.
--Pharmacy --Microbiology and Immunology