Detecting Plasmodium Sp and Arboviruses, All in One Test
P Koliopoulos
Detecting Plasmodium Sp and Arboviruses, All in One Test - Mwanza, Tanzania: Romanian Archives of Microbiology and Immunology & Catholic University of Health and Allied Sciences [CUHAS – Bugando] 2021 - Romanian Archives of Microbiology and Immunology; Bucharest Vol. 80, Iss. 3, (Jul-Sep 2021): 298. .
The current study evaluates the ability of a multiplex-reverse transcriptase-polymerase chain reaction test combined with an enzyme linked immunosorbent assay (multiplex-RT-PCR-ELISA) to identify and differentiate nine human mosquitoborne pathogens (eight arboviruses and Plasmodium sp.). Venous blood samples were collected from 132 children (ranging from 5-months to 12-years) who presented with acute high-grade fever (> 38.0 C) and met the WHO criteria of a presumptive malaria infection, to a medical center in Tanzania, between April 2016 and October 2016. Serial dilutions of a standard Zika virus suspension (1x107 viral RNA copies/ml) were added to the master mix containing the 9 primer sets.
Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz
Detecting Plasmodium Sp and Arboviruses, All in One Test - Mwanza, Tanzania: Romanian Archives of Microbiology and Immunology & Catholic University of Health and Allied Sciences [CUHAS – Bugando] 2021 - Romanian Archives of Microbiology and Immunology; Bucharest Vol. 80, Iss. 3, (Jul-Sep 2021): 298. .
The current study evaluates the ability of a multiplex-reverse transcriptase-polymerase chain reaction test combined with an enzyme linked immunosorbent assay (multiplex-RT-PCR-ELISA) to identify and differentiate nine human mosquitoborne pathogens (eight arboviruses and Plasmodium sp.). Venous blood samples were collected from 132 children (ranging from 5-months to 12-years) who presented with acute high-grade fever (> 38.0 C) and met the WHO criteria of a presumptive malaria infection, to a medical center in Tanzania, between April 2016 and October 2016. Serial dilutions of a standard Zika virus suspension (1x107 viral RNA copies/ml) were added to the master mix containing the 9 primer sets.
Phone: +255 28 298 3384 Fax: +255 28 298 3386 Email: vc@bugando.ac.tz Website: www.bugando.ac.tz